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. 2004 Jun;13(3):171–180. doi: 10.1080/09511920410001713538

Clinical significance of circulating dendritic cells in patients with systemic lupus erythematosus.

E Robak 1, P Smolewski 1, A Woźniacka 1, A Sysa-Jedrzejowska 1, T Robak 1
PMCID: PMC1781551  PMID: 15223608

Abstract

Dendritic cells are a complex group of mainly bone-marrow-derived leukocytes that play a role in autoimmune diseases. The total number of circulating dendritic cells (tDC), and their plasmacytoid dendritic cell (pDC) and myeloid dendritic cell (mDC1 and mDC2) subpopulations were assessed using flow cytometry. The number of tDC and their subsets were significantly lower in systemic lupus erythematosus patients than in the control group. The count of tDC and their subsets correlated with the number of T cells. The number of tDC and pDC subpopulation were lower in the patients with lymphopenia and leukopenia than in the patients without these symptoms. Our data suggest that fluctuations in blood dendritic cell count in systemic lupus erythematosus patients are much more significant in pDC than in mDC, what may be caused by their migration to the sites of inflammation including skin lesions. Positive correlation between dendritic cell number and TCD4+, TCD8+ and CD19+ B cells, testify of their interactions and influence on SLE pathogenesis. The association between dendritic cell number and clinical features seems to be less clear.

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Selected References

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  1. Banchereau J., Briere F., Caux C., Davoust J., Lebecque S., Liu Y. J., Pulendran B., Palucka K. Immunobiology of dendritic cells. Annu Rev Immunol. 2000;18:767–811. doi: 10.1146/annurev.immunol.18.1.767. [DOI] [PubMed] [Google Scholar]
  2. Blanco P., Palucka A. K., Gill M., Pascual V., Banchereau J. Induction of dendritic cell differentiation by IFN-alpha in systemic lupus erythematosus. Science. 2001 Nov 16;294(5546):1540–1543. doi: 10.1126/science.1064890. [DOI] [PubMed] [Google Scholar]
  3. Blanco P., Palucka A. K., Gill M., Pascual V., Banchereau J. Induction of dendritic cell differentiation by IFN-alpha in systemic lupus erythematosus. Science. 2001 Nov 16;294(5546):1540–1543. doi: 10.1126/science.1064890. [DOI] [PubMed] [Google Scholar]
  4. Blomberg S., Eloranta M. L., Cederblad B., Nordlin K., Alm G. V., Rönnblom L. Presence of cutaneous interferon-alpha producing cells in patients with systemic lupus erythematosus. Lupus. 2001;10(7):484–490. doi: 10.1191/096120301678416042. [DOI] [PubMed] [Google Scholar]
  5. Blomberg Stina, Eloranta Maija-Leena, Magnusson Mattias, Alm Gunnar V., Rönnblom Lars. Expression of the markers BDCA-2 and BDCA-4 and production of interferon-alpha by plasmacytoid dendritic cells in systemic lupus erythematosus. Arthritis Rheum. 2003 Sep;48(9):2524–2532. doi: 10.1002/art.11225. [DOI] [PubMed] [Google Scholar]
  6. Caux C., Massacrier C., Vanbervliet B., Dubois B., Durand I., Cella M., Lanzavecchia A., Banchereau J. CD34+ hematopoietic progenitors from human cord blood differentiate along two independent dendritic cell pathways in response to granulocyte-macrophage colony-stimulating factor plus tumor necrosis factor alpha: II. Functional analysis. Blood. 1997 Aug 15;90(4):1458–1470. [PubMed] [Google Scholar]
  7. Cella M., Jarrossay D., Facchetti F., Alebardi O., Nakajima H., Lanzavecchia A., Colonna M. Plasmacytoid monocytes migrate to inflamed lymph nodes and produce large amounts of type I interferon. Nat Med. 1999 Aug;5(8):919–923. doi: 10.1038/11360. [DOI] [PubMed] [Google Scholar]
  8. Converso M., Bertero M. T., Vallario A., Caligaris-Cappio F. Analysis of T-cell clones in systemic lupus erythematosus. Haematologica. 2000 Feb;85(2):118–123. [PubMed] [Google Scholar]
  9. Cravens Petra D., Lipsky Peter E. Dendritic cells, chemokine receptors and autoimmune inflammatory diseases. Immunol Cell Biol. 2002 Oct;80(5):497–505. doi: 10.1046/j.1440-1711.2002.01118.x. [DOI] [PubMed] [Google Scholar]
  10. Darmochwal-Kolarz D., Rolinski J., Tabarkiewicz J., Leszczynska-Gorzelak B., Buczkowski J., Wojas K., Oleszczuk J. Blood myeloid and lymphoid dendritic cells are stable during the menstrual cycle but deficient during mid-gestation. J Reprod Immunol. 2003 Aug;59(2):193–203. doi: 10.1016/s0165-0378(03)00047-0. [DOI] [PubMed] [Google Scholar]
  11. Dzionek A., Fuchs A., Schmidt P., Cremer S., Zysk M., Miltenyi S., Buck D. W., Schmitz J. BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood. J Immunol. 2000 Dec 1;165(11):6037–6046. doi: 10.4049/jimmunol.165.11.6037. [DOI] [PubMed] [Google Scholar]
  12. Dzionek A., Sohma Y., Nagafune J., Cella M., Colonna M., Facchetti F., Günther G., Johnston I., Lanzavecchia A., Nagasaka T. BDCA-2, a novel plasmacytoid dendritic cell-specific type II C-type lectin, mediates antigen capture and is a potent inhibitor of interferon alpha/beta induction. J Exp Med. 2001 Dec 17;194(12):1823–1834. doi: 10.1084/jem.194.12.1823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Emlen W., Niebur J., Kadera R. Accelerated in vitro apoptosis of lymphocytes from patients with systemic lupus erythematosus. J Immunol. 1994 Apr 1;152(7):3685–3692. [PubMed] [Google Scholar]
  14. Farkas L., Beiske K., Lund-Johansen F., Brandtzaeg P., Jahnsen F. L. Plasmacytoid dendritic cells (natural interferon- alpha/beta-producing cells) accumulate in cutaneous lupus erythematosus lesions. Am J Pathol. 2001 Jul;159(1):237–243. doi: 10.1016/s0002-9440(10)61689-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gabrilovich D. I., Corak J., Ciernik I. F., Kavanaugh D., Carbone D. P. Decreased antigen presentation by dendritic cells in patients with breast cancer. Clin Cancer Res. 1997 Mar;3(3):483–490. [PubMed] [Google Scholar]
  16. Gill Michelle A., Blanco Patrick, Arce Edsel, Pascual Virginia, Banchereau Jacques, Palucka A. Karolina. Blood dendritic cells and DC-poietins in systemic lupus erythematosus. Hum Immunol. 2002 Dec;63(12):1172–1180. doi: 10.1016/s0198-8859(02)00756-5. [DOI] [PubMed] [Google Scholar]
  17. Gilliet Michel, Liu Yong-Jun. Generation of human CD8 T regulatory cells by CD40 ligand-activated plasmacytoid dendritic cells. J Exp Med. 2002 Mar 18;195(6):695–704. doi: 10.1084/jem.20011603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Grouard G., Rissoan M. C., Filgueira L., Durand I., Banchereau J., Liu Y. J. The enigmatic plasmacytoid T cells develop into dendritic cells with interleukin (IL)-3 and CD40-ligand. J Exp Med. 1997 Mar 17;185(6):1101–1111. doi: 10.1084/jem.185.6.1101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Hochberg M. C. Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1997 Sep;40(9):1725–1725. doi: 10.1002/art.1780400928. [DOI] [PubMed] [Google Scholar]
  20. Jahnsen F. L., Lund-Johansen F., Dunne J. F., Farkas L., Haye R., Brandtzaeg P. Experimentally induced recruitment of plasmacytoid (CD123high) dendritic cells in human nasal allergy. J Immunol. 2000 Oct 1;165(7):4062–4068. doi: 10.4049/jimmunol.165.7.4062. [DOI] [PubMed] [Google Scholar]
  21. Liang M. H., Socher S. A., Larson M. G., Schur P. H. Reliability and validity of six systems for the clinical assessment of disease activity in systemic lupus erythematosus. Arthritis Rheum. 1989 Sep;32(9):1107–1118. doi: 10.1002/anr.1780320909. [DOI] [PubMed] [Google Scholar]
  22. Liu Y. J. Dendritic cell subsets and lineages, and their functions in innate and adaptive immunity. Cell. 2001 Aug 10;106(3):259–262. doi: 10.1016/s0092-8674(01)00456-1. [DOI] [PubMed] [Google Scholar]
  23. Lorenz H. M., Grünke M., Hieronymus T., Herrmann M., Kühnel A., Manger B., Kalden J. R. In vitro apoptosis and expression of apoptosis-related molecules in lymphocytes from patients with systemic lupus erythematosus and other autoimmune diseases. Arthritis Rheum. 1997 Feb;40(2):306–317. doi: 10.1002/art.1780400216. [DOI] [PubMed] [Google Scholar]
  24. MacDonald Kelli P. A., Munster David J., Clark Georgina J., Dzionek Andrzej, Schmitz Juergen, Hart Derek N. J. Characterization of human blood dendritic cell subsets. Blood. 2002 Aug 15;100(13):4512–4520. doi: 10.1182/blood-2001-11-0097. [DOI] [PubMed] [Google Scholar]
  25. Mills J. A. Systemic lupus erythematosus. N Engl J Med. 1994 Jun 30;330(26):1871–1879. doi: 10.1056/NEJM199406303302608. [DOI] [PubMed] [Google Scholar]
  26. Mok C. C., Lau C. S. Pathogenesis of systemic lupus erythematosus. J Clin Pathol. 2003 Jul;56(7):481–490. doi: 10.1136/jcp.56.7.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. O'Doherty U., Peng M., Gezelter S., Swiggard W. J., Betjes M., Bhardwaj N., Steinman R. M. Human blood contains two subsets of dendritic cells, one immunologically mature and the other immature. Immunology. 1994 Jul;82(3):487–493. [PMC free article] [PubMed] [Google Scholar]
  28. Palucka A. Karolina, Banchereau Jacques, Blanco Patrick, Pascual Virginia. The interplay of dendritic cell subsets in systemic lupus erythematosus. Immunol Cell Biol. 2002 Oct;80(5):484–488. doi: 10.1046/j.1440-1711.2002.01112.x. [DOI] [PubMed] [Google Scholar]
  29. Pascual Virginia, Banchereau Jacques, Palucka A. Karolina. The central role of dendritic cells and interferon-alpha in SLE. Curr Opin Rheumatol. 2003 Sep;15(5):548–556. doi: 10.1097/00002281-200309000-00005. [DOI] [PubMed] [Google Scholar]
  30. Patterson S., Helbert M., English N. R., Pinching A. J., Knight S. C. The effect of AZT on dendritic cell number and provirus load in the peripheral blood of AIDS patients: a preliminary study. Res Virol. 1996 Mar-Jun;147(2-3):109–114. doi: 10.1016/0923-2516(96)80224-x. [DOI] [PubMed] [Google Scholar]
  31. Robak E., Błoński J. Z., Bartkowiak J., Niewiadomska H., Sysa-Jedrzejowska A., Robak T. Circulating TCR gammadelta cells in the patients with systemic lupus erythematosus. Mediators Inflamm. 1999;8(6):305–312. doi: 10.1080/09629359990315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Robak E., Woźniacka A., Sysa-Jedrzejowska A., Stepień H., Robak T. Circulating angiogenesis inhibitor endostatin and positive endothelial growth regulators in patients with systemic lupus erythematosus. Lupus. 2002;11(6):348–355. doi: 10.1191/0961203302lu199oa. [DOI] [PubMed] [Google Scholar]
  33. Scheinecker C., Zwölfer B., Köller M., Männer G., Smolen J. S. Alterations of dendritic cells in systemic lupus erythematosus: phenotypic and functional deficiencies. Arthritis Rheum. 2001 Apr;44(4):856–865. doi: 10.1002/1529-0131(200104)44:4<856::AID-ANR142>3.0.CO;2-A. [DOI] [PubMed] [Google Scholar]
  34. Shodell M., Shah K., Siegal F. P. Circulating human plasmacytoid dendritic cells are highly sensitive to corticosteroid administration. Lupus. 2003;12(3):222–230. doi: 10.1191/0961203303lu362xx. [DOI] [PubMed] [Google Scholar]
  35. Siegal F. P., Kadowaki N., Shodell M., Fitzgerald-Bocarsly P. A., Shah K., Ho S., Antonenko S., Liu Y. J. The nature of the principal type 1 interferon-producing cells in human blood. Science. 1999 Jun 11;284(5421):1835–1837. doi: 10.1126/science.284.5421.1835. [DOI] [PubMed] [Google Scholar]
  36. Siegal F. P., Kadowaki N., Shodell M., Fitzgerald-Bocarsly P. A., Shah K., Ho S., Antonenko S., Liu Y. J. The nature of the principal type 1 interferon-producing cells in human blood. Science. 1999 Jun 11;284(5421):1835–1837. doi: 10.1126/science.284.5421.1835. [DOI] [PubMed] [Google Scholar]
  37. Swaak A. J., Nossent J. C., Smeenk R. J. Systemic lupus erythematosus. Int J Clin Lab Res. 1992;22(4):190–195. doi: 10.1007/BF02591422. [DOI] [PubMed] [Google Scholar]
  38. Upham J. W., Lundahl J., Liang H., Denburg J. A., O'Byrne P. M., Snider D. P. Simplified quantitation of myeloid dendritic cells in peripheral blood using flow cytometry. Cytometry. 2000 May 1;40(1):50–59. doi: 10.1002/(sici)1097-0320(20000501)40:1<50::aid-cyto7>3.0.co;2-p. [DOI] [PubMed] [Google Scholar]

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