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. 1996 Jul;178(14):4039–4046. doi: 10.1128/jb.178.14.4039-4046.1996

Engineering hybrid pseudomonads capable of utilizing a wide range of aromatic hydrocarbons and of efficient degradation of trichloroethylene.

A Suyama 1, R Iwakiri 1, N Kimura 1, A Nishi 1, K Nakamura 1, K Furukawa 1
PMCID: PMC178158  PMID: 8763929

Abstract

We constructed hybrid Pseudomonas strains in which the bphA1 gene (coding for a large subunit of biphenyl dioxygenase) is replaced with the todC1 gene (coding for a large subunit of toluene dioxygenase of Pseudomonas putida Fl) within chromosomal biphenyl-catabolic bph gene clusters. Such hybrid strains gained the novel capability to grow on a wide range of aromatic hydrocarbons, and, more interestingly, they degraded chloroethenes such as trichloroethylene and cis-1,2-dichloroethylene very efficiently.

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Selected References

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