Abstract
BACKGROUND: Mylar balloons are used to collect exhaled air for analysis of fractional nitric oxide concentration (FENO). AIM: We studied the effect of storage conditions on the stability of nitric oxide (NO) in mylar balloons. METHODS: Exhaled air samples and calibration gases were stored in mylar balloons at 4, 21 and 37 degrees C, with or without silica gel. NO was measured after 0, 6, 9, 24 and 48 h. Scheffe F-tests were used to compare NO values. RESULTS: NO remained stable in balloons for 9 h at all temperatures, without silica gel. NO increased between 9 and 48 h, but only with low initial FENO. Silica gel increased variability. CONCLUSIONS: FENO in mylar balloons is stable for at least 9 h. The storage temperature is not critical, but silica gel increases variability.
Full Text
The Full Text of this article is available as a PDF (165.5 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baraldi E., de Jongste J. C., European Respiratory Society/American Thoracic Society (ERS/ATS) Task Force Measurement of exhaled nitric oxide in children, 2001. Eur Respir J. 2002 Jul;20(1):223–237. doi: 10.1183/09031936.02.00293102. [DOI] [PubMed] [Google Scholar]
- Barreto M., Villa M. P., Martella S., Falasca C., Guglielmi F., Pagani J., Darder M. T., Ronchetti R. Off-line exhaled nitric oxide measurements in children. Pediatr Pulmonol. 2001 Aug;32(2):159–167. doi: 10.1002/ppul.1102. [DOI] [PubMed] [Google Scholar]
- Jöbsis Q., Raatgeep H. C., Hop W. C., de Jongste J. C. Controlled low flow off line sampling of exhaled nitric oxide in children. Thorax. 2001 Apr;56(4):285–289. doi: 10.1136/thorax.56.4.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jöbsis Q., Schellekens S. L., Kroesbergen A., Hop W. C., de Jongste J. C. Sampling of exhaled nitric oxide in children: end-expiratory plateau, balloon and tidal breathing methods compared. Eur Respir J. 1999 Jun;13(6):1406–1410. doi: 10.1183/09031936.99.13614119. [DOI] [PubMed] [Google Scholar]
- Kharitonov S. A., Barnes P. J. Exhaled markers of pulmonary disease. Am J Respir Crit Care Med. 2001 Jun;163(7):1693–1722. doi: 10.1164/ajrccm.163.7.2009041. [DOI] [PubMed] [Google Scholar]
- Paredi P., Loukides S., Ward S., Cramer D., Spicer M., Kharitonov S. A., Barnes P. J. Exhalation flow and pressure-controlled reservoir collection of exhaled nitric oxide for remote and delayed analysis. Thorax. 1998 Sep;53(9):775–779. doi: 10.1136/thx.53.9.775. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pijnenburg M. W. H., Lissenberg E. T., Hofhuis W., Ghiro L., Ho W. C. J., Holland W. P., de Jongste J. C. Exhaled nitric oxide measurements with dynamic flow restriction in children aged 4-8 yrs. Eur Respir J. 2002 Oct;20(4):919–924. doi: 10.1183/09031936.02.01282002. [DOI] [PubMed] [Google Scholar]
- Silkoff P. E., Stevens A., Pak J., Bucher-Bartelson B., Martin R. J. A method for the standardized offline collection of exhaled nitric oxide. Chest. 1999 Sep;116(3):754–759. doi: 10.1378/chest.116.3.754. [DOI] [PubMed] [Google Scholar]