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. 2003 Oct;12(5):303–307. doi: 10.1080/09629350310001619735

Tumour necrosis factor-alpha polymorphism as one of the complex inherited factors in pemphigus.

Jolanta Dorota Torzecka 1, Joanna Narbutt 1, Anna Sysa-Jedrzejowska 1, Maciej Borowiec 1, Anetta Ptasinska 1, Grzegorz Woszczek 1, Marek L Kowalski 1
PMCID: PMC1781627  PMID: 14760938

Abstract

The aim of our study was to analyse a significance of tumour necrosis factor (TNF)-alpha promoter gene polymorphisms in relation to the HLA-DR locus in genetic predisposition to pemphigus. TNF-alpha gene polymorphisms in position -238 and -308 were identified using a modified polymerase chain reaction-restriction fragment length polymorphism method in 53 patients with pemphigus (38 with pemphigus vulgaris, 15 with pemphigus foliaceus) and 87 healthy controls. The HLA-DRB1 locus was typed using the polymerase chain reaction SSO method in all the patients and 152 population controls. Carriers of the TNF-alpha polymorphic -308 A allele were found to be more frequent in the pemphigus foliaceus group in comparison with the control group (odds ratio (OR) = 8.12; p = 0.0005). A significant association between HLA-DRB1*04 (OR = 3.86; pcor = 0.0001) and DRB1*14 (OR = 8.4; pcor = 0.0001) and pemphigus vulgaris was found. In this group of patients a decreased frequency of HLA-DRB1*07 (OR = 0.08; pcor = 0.006) was also identified. We have shown for the first time a positive association of TNF-alpha polymorphism in position -308 with pemphigus foliaceus.

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Selected References

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