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. 2002 Apr;11(2):105–111. doi: 10.1080/09629350220131962

Cytokine secretion by decidual lymphocytes in transient hypertension of pregnancy and pre-eclampsia.

Jacek R Wilczyński 1, Henryk Tchórzewski 1, Ewa Głowacka 1, Małgorzata Banasik 1, Przemyslaw Lewkowicz 1, Marian Szpakowski 1, Krzysztof Zeman 1, Jan Wilczyński 1
PMCID: PMC1781645  PMID: 12061422

Abstract

BACKGROUND: Transient hypertension (TH) and preeclampsia (PE) are believed to have different pathophysiology. However, 15-25% of pregnant women initially diagnosed as having TH develop PE. To clarify the immuno-pathogenetical connections between the two syndromes, we studied the pattern of T helper cell (Th)1/Th2 cytokine balance disturbances existing inside maternal decidua in normal pregnancy (NP) and pregnancies complicated with TH and PE. METHODS: Third-trimester decidual tissue was obtained by curettage of uterine cavity during elective caesarean sections in NP (n = 11), TH (n = 17) and PE (n = 21) patients. Cell suspensions were prepared by an electromechanical dispersal method and centrifugated using a standard gradient sedimentation technique. Isolated lymphocytes were placed in medium (RPMI 1640, 10% fetal calf serum, L-glutamine, penicillin, streptomycin) and cultured for 72 h with or without mitogen phytohaemaglutinine (PHA). The enzyme-linked immunosorbent assay method was used for estimation of interleukin (IL)-2, IL-4, IL-6, IL-10, IL-12 and interferon-gamma (IFN-gamma) in culture supernatant. STATISTICAL ANALYSIS: The Kruskal-Wallis and the Mann-Whitney U tests were used (p < 0.05). RESULTS: Both spontaneous and PHA-stimulated secretion of Th2-type cytokines IL-6 and IL-10 was decreased in PE patients compared with TH and NP patients. The concentration of Th1-type cytokine IFN-gamma was increased in patients suffering both from TH and PE. CONCLUSION: On the base of decidual cytokine secretion, both PE and TH are syndromes of local Th1/Th2 cytokine balance disturbances as compared with NP, and TH seems to be an intermediate step to PE.

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Selected References

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  1. Belardelli F. Role of interferons and other cytokines in the regulation of the immune response. APMIS. 1995 Mar;103(3):161–179. doi: 10.1111/j.1699-0463.1995.tb01092.x. [DOI] [PubMed] [Google Scholar]
  2. Bennett W. A., Lagoo-Deenadayalan S., Brackin M. N., Hale E., Cowan B. D. Cytokine expression by models of human trophoblast as assessed by a semiquantitative reverse transcription-polymerase chain reaction technique. Am J Reprod Immunol. 1996 Nov;36(5):285–294. doi: 10.1111/j.1600-0897.1996.tb00178.x. [DOI] [PubMed] [Google Scholar]
  3. Chaouat G., Tranchot Diallo J., Volumenie J. L., Menu E., Gras G., Delage G., Mognetti B. Immune suppression and Th1/Th2 balance in pregnancy revisited: a (very) personal tribute to Tom Wegmann. Am J Reprod Immunol. 1997 Jun;37(6):427–434. doi: 10.1111/j.1600-0897.1997.tb00255.x. [DOI] [PubMed] [Google Scholar]
  4. Clark D. A., Arck P. C., Jalali R., Merali F. S., Manuel J., Chaouat G., Underwood J. L., Mowbray J. F. Psycho-neuro-cytokine/endocrine pathways in immunoregulation during pregnancy. Am J Reprod Immunol. 1996 Apr;35(4):330–337. doi: 10.1111/j.1600-0897.1996.tb00489.x. [DOI] [PubMed] [Google Scholar]
  5. Clark D. A., Chaouat G., Arck P. C., Mittruecker H. W., Levy G. A. Cytokine-dependent abortion in CBA x DBA/2 mice is mediated by the procoagulant fgl2 prothrombinase [correction of prothombinase]. J Immunol. 1998 Jan 15;160(2):545–549. [PubMed] [Google Scholar]
  6. Colbern G. T., Chiang M. H., Main E. K. Expression of the nonclassic histocompatibility antigen HLA-G by preeclamptic placenta. Am J Obstet Gynecol. 1994 May;170(5 Pt 1):1244–1250. doi: 10.1016/s0002-9378(94)70134-2. [DOI] [PubMed] [Google Scholar]
  7. Conrad K. P., Miles T. M., Benyo D. F. Circulating levels of immunoreactive cytokines in women with preeclampsia. Am J Reprod Immunol. 1998 Aug;40(2):102–111. doi: 10.1111/j.1600-0897.1998.tb00398.x. [DOI] [PubMed] [Google Scholar]
  8. D'Anna R., Baviera G., Scilipoti A., Leonardi I., Leo R. The clinical utility of serum uric acid measurements in pre-eclampsia and transient hypertension in pregnancy. Panminerva Med. 2000 Jun;42(2):101–103. [PubMed] [Google Scholar]
  9. Daniel Y., Kupferminc M. J., Baram A., Jaffa A. J., Fait G., Wolman I., Lessing J. B. Plasma interleukin-12 is elevated in patients with preeclampsia. Am J Reprod Immunol. 1998 Jun;39(6):376–380. doi: 10.1111/j.1600-0897.1998.tb00372.x. [DOI] [PubMed] [Google Scholar]
  10. Darmochwal-Kolarz D., Leszczynska-Gorzelak B., Rolinski J., Oleszczuk J. T helper 1- and T helper 2-type cytokine imbalance in pregnant women with pre-eclampsia. Eur J Obstet Gynecol Reprod Biol. 1999 Oct;86(2):165–170. doi: 10.1016/s0301-2115(99)00065-2. [DOI] [PubMed] [Google Scholar]
  11. Del Prete G., Maggi E., Romagnani S. Human Th1 and Th2 cells: functional properties, mechanisms of regulation, and role in disease. Lab Invest. 1994 Mar;70(3):299–306. [PubMed] [Google Scholar]
  12. Hennessy A., Pilmore H. L., Simmons L. A., Painter D. M. A deficiency of placental IL-10 in preeclampsia. J Immunol. 1999 Sep 15;163(6):3491–3495. [PubMed] [Google Scholar]
  13. Higgins J. R., Papayianni A., Brady H. R., Darling M. R., Walshe J. J. Circulating vascular cell adhesion molecule-1 in pre-eclampsia, gestational hypertension, and normal pregnancy: evidence of selective dysregulation of vascular cell adhesion molecule-1 homeostasis in pre-eclampsia. Am J Obstet Gynecol. 1998 Aug;179(2):464–469. doi: 10.1016/s0002-9378(98)70380-1. [DOI] [PubMed] [Google Scholar]
  14. Kauma S. W., Wang Y., Walsh S. W. Preeclampsia is associated with decreased placental interleukin-6 production. J Soc Gynecol Investig. 1995 Jul-Aug;2(4):614–617. doi: 10.1016/1071-5576(95)00007-2. [DOI] [PubMed] [Google Scholar]
  15. Krauss T., Kuhn W., Lakoma C., Augustin H. G. Circulating endothelial cell adhesion molecules as diagnostic markers for the early identification of pregnant women at risk for development of preeclampsia. Am J Obstet Gynecol. 1997 Aug;177(2):443–449. doi: 10.1016/s0002-9378(97)70213-8. [DOI] [PubMed] [Google Scholar]
  16. Kuwajima T., Suzuki S., Yoneyama Y., Sawa R., Asakura H., Araki T. Relation between plasma endothelin 1 levels and T helper 1: T helper 2 cell immunity in women with preeclampsia. Gynecol Obstet Invest. 2001;52(4):260–263. doi: 10.1159/000052987. [DOI] [PubMed] [Google Scholar]
  17. Luppi P., McKnight C., Mathie T., Faas S., Rudert W. A., Stewart-Akers A. M., Trucco M., DeLoia J. A. Evidence for superantigen involvement in preeclampsia. Am J Reprod Immunol. 2000 Apr;43(4):187–196. doi: 10.1111/j.8755-8920.2000.430401.x. [DOI] [PubMed] [Google Scholar]
  18. Marzi M., Vigano A., Trabattoni D., Villa M. L., Salvaggio A., Clerici E., Clerici M. Characterization of type 1 and type 2 cytokine production profile in physiologic and pathologic human pregnancy. Clin Exp Immunol. 1996 Oct;106(1):127–133. doi: 10.1046/j.1365-2249.1996.d01-809.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Matthiesen L., Berg G., Ernerudh J., Håkansson L. Lymphocyte subsets and mitogen stimulation of blood lymphocytes in preeclampsia. Am J Reprod Immunol. 1999 Mar;41(3):192–203. doi: 10.1111/j.1600-0897.1999.tb00532.x. [DOI] [PubMed] [Google Scholar]
  20. McRae B. L., Semnani R. T., Hayes M. P., van Seventer G. A. Type I IFNs inhibit human dendritic cell IL-12 production and Th1 cell development. J Immunol. 1998 May 1;160(9):4298–4304. [PubMed] [Google Scholar]
  21. Piccinni M. P., Maggi E., Romagnani S. Role of hormone-controlled T-cell cytokines in the maintenance of pregnancy. Biochem Soc Trans. 2000 Feb;28(2):212–215. doi: 10.1042/bst0280212. [DOI] [PubMed] [Google Scholar]
  22. Sacks G., Sargent I., Redman C. An innate view of human pregnancy. Immunol Today. 1999 Mar;20(3):114–118. doi: 10.1016/s0167-5699(98)01393-0. [DOI] [PubMed] [Google Scholar]
  23. Saito S., Sakai M., Sasaki Y., Tanebe K., Tsuda H., Michimata T. Quantitative analysis of peripheral blood Th0, Th1, Th2 and the Th1:Th2 cell ratio during normal human pregnancy and preeclampsia. Clin Exp Immunol. 1999 Sep;117(3):550–555. doi: 10.1046/j.1365-2249.1999.00997.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Saudan P., Brown M. A., Buddle M. L., Jones M. Does gestational hypertension become pre-eclampsia? Br J Obstet Gynaecol. 1998 Nov;105(11):1177–1184. doi: 10.1111/j.1471-0528.1998.tb09971.x. [DOI] [PubMed] [Google Scholar]
  25. Stallmach T., Hebisch G., Orban P., Lü X. Aberrant positioning of trophoblast and lymphocytes in the feto-maternal interface with pre-eclampsia. Virchows Arch. 1999 Mar;434(3):207–211. doi: 10.1007/s004280050329. [DOI] [PubMed] [Google Scholar]
  26. Steinborn A., Sohn C., Sayehli C., Niederhut A., Schmitt E., Kaufmann M. Preeclampsia, a pregnancy-specific disease, is associated with fetal monocyte activation. Clin Immunol. 2001 Sep;100(3):305–313. doi: 10.1006/clim.2001.5081. [DOI] [PubMed] [Google Scholar]
  27. Szekeres-Bartho J., Faust Z., Varga P., Szereday L., Kelemen K. The immunological pregnancy protective effect of progesterone is manifested via controlling cytokine production. Am J Reprod Immunol. 1996 Apr;35(4):348–351. doi: 10.1111/j.1600-0897.1996.tb00492.x. [DOI] [PubMed] [Google Scholar]
  28. Tchórzewski H., Krasomski G., Biesiada L., Głowacka E., Banasik M., Lewkowicz P. IL-12, IL-6 and IFN-gamma production by lymphocytes of pregnant women with rheumatoid arthritis remission during pregnancy. Mediators Inflamm. 2000;9(6):289–293. doi: 10.1080/09629350020027609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Terrone D. A., Rinehart B. K., May W. L., Martin R. W., Martin J. N., Jr The myth of transient hypertension: descriptor or disease process? Am J Perinatol. 2001;18(2):73–77. doi: 10.1055/s-2001-13635. [DOI] [PubMed] [Google Scholar]
  30. Tranchot-Diallo J., Gras G., Parnet-Mathieu F., Benveniste O., Marcé D., Roques P., Milliez J., Chaouat G., Dormont D. Modulations of cytokine expression in pregnant women. Am J Reprod Immunol. 1997 Mar;37(3):215–226. doi: 10.1111/j.1600-0897.1997.tb00218.x. [DOI] [PubMed] [Google Scholar]
  31. Trinchieri G. Interleukin-12 and its role in the generation of TH1 cells. Immunol Today. 1993 Jul;14(7):335–338. doi: 10.1016/0167-5699(93)90230-I. [DOI] [PubMed] [Google Scholar]
  32. Wegmann T. G., Lin H., Guilbert L., Mosmann T. R. Bidirectional cytokine interactions in the maternal-fetal relationship: is successful pregnancy a TH2 phenomenon? Immunol Today. 1993 Jul;14(7):353–356. doi: 10.1016/0167-5699(93)90235-D. [DOI] [PubMed] [Google Scholar]
  33. Zamorski M. A., Green L. A. NHBPEP report on high blood pressure in pregnancy: a summary for family physicians. Am Fam Physician. 2001 Jul 15;64(2):263-70, 216. [PubMed] [Google Scholar]
  34. Zamorski M. A., Green L. A. Preeclampsia and hypertensive disorders of pregnancy. Am Fam Physician. 1996 Apr;53(5):1595–1610. [PubMed] [Google Scholar]

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