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. 2001 Jun;10(3):143–154. doi: 10.1080/09629350124877

Inflammatory cell distribution in guinea pig airways and its relationship to airway reactivity.

F Westerhof 1, W Timens 1, A van Oosten 1, A B Zuidhof 1, N Nauta 1, M Schuiling 1, J T Vos 1, J Zaagsma 1, H Meurs 1, W Coers 1
PMCID: PMC1781701  PMID: 11545251

Abstract

Although airway inflammation and airway hyperreactivity are observed after allergen inhalation both in allergic humans and animals, little is known about the mechanisms by which inflammatory cells can contribute to allergen-induced airway hyperreactivity. To understand how inflammatory cell infiltration can contribute to airway hyperreactivity, the location of these cells within the airways may be crucial Using a guinea pig model of acute allergic asthma, we investigated the inflammatory cell infiltration in different airway compartments at 6 and 24 h (i.e. after the early and the late asthmatic reaction, respectively) after allergen or saline challenge in relation to changes in airway reactivity (AR) to histamine. At 6 h after allergen challenge, a threefold (p < 0.01) increase in the AR to histamine was observed. At 24 h after challenge, the AR to histamine was lower, but still significantly enhanced (1.6-fold, p < 0.05). Adventitial eosinophil and neutrophil numbers in both bronchi and bronchioli were significantly increased at 6 h post-allergen provocation as compared with saline (p < 0.01 for all), while there was a strong tendency to enhanced eosinophils in the bronchial submucosa at this time point (p = 0.08). At 24h after allergen challenge, the eosinophilic and neutrophilic cell infiltration was reduced. CD3+ T lymphocytes were increased in the adventitial compartment of the large airways (p < 0.05) and in the parenchyma (p < 0.05) at 24h post-allergen, while numbers of CD8+ cells did not differ from saline treatment at any time point post-provocation. The results indicate that, after allergen provocation, inflammatory cell numbers in the airways are mainly elevated in the adventitial compartment. The adventitial inflammation could be important for the development of allergen-induced airway hyperreactivity.

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Selected References

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  1. Aalbers R., Kauffman H. F., Groen H., Koëter G. H., de Monchy J. G. The effect of nedocromil sodium on the early and late reaction and allergen-induced bronchial hyperresponsiveness. J Allergy Clin Immunol. 1991 May;87(5):993–1001. doi: 10.1016/0091-6749(91)90422-k. [DOI] [PubMed] [Google Scholar]
  2. Aalbers R., Kauffman H. F., Vrugt B., Koëter G. H., de Monchy J. G. Allergen-induced recruitment of inflammatory cells in lavage 3 and 24 h after challenge in allergic asthmatic lungs. Chest. 1993 Apr;103(4):1178–1184. doi: 10.1378/chest.103.4.1178. [DOI] [PubMed] [Google Scholar]
  3. Aalbers R., de Monchy J. G., Kauffman H. F., Smith M., Hoekstra Y., Vrugt B., Timens W. Dynamics of eosinophil infiltration in the bronchial mucosa before and after the late asthmatic reaction. Eur Respir J. 1993 Jun;6(6):840–847. [PubMed] [Google Scholar]
  4. Beasley R., Roche W. R., Roberts J. A., Holgate S. T. Cellular events in the bronchi in mild asthma and after bronchial provocation. Am Rev Respir Dis. 1989 Mar;139(3):806–817. doi: 10.1164/ajrccm/139.3.806. [DOI] [PubMed] [Google Scholar]
  5. Bentley A. M., Menz G., Storz C., Robinson D. S., Bradley B., Jeffery P. K., Durham S. R., Kay A. B. Identification of T lymphocytes, macrophages, and activated eosinophils in the bronchial mucosa in intrinsic asthma. Relationship to symptoms and bronchial responsiveness. Am Rev Respir Dis. 1992 Aug;146(2):500–506. doi: 10.1164/ajrccm/146.2.500. [DOI] [PubMed] [Google Scholar]
  6. Boer J., Duyvendak M., Schuurman F. E., Pouw F. M., Zaagsma J., Meurs H. Role of L-arginine in the deficiency of nitric oxide and airway hyperreactivity after the allergen-induced early asthmatic reaction in guinea-pigs. Br J Pharmacol. 1999 Nov;128(5):1114–1120. doi: 10.1038/sj.bjp.0702882. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Boichot E., Lagente V., Carre C., Waltmann P., Mencia-Huerta J. M., Braquet P. Bronchial hyperresponsiveness and cellular infiltration in the lung of guinea-pigs sensitized and challenged by aerosol. Clin Exp Allergy. 1991 Jan;21(1):67–76. doi: 10.1111/j.1365-2222.1991.tb00806.x. [DOI] [PubMed] [Google Scholar]
  8. Bradley B. L., Azzawi M., Jacobson M., Assoufi B., Collins J. V., Irani A. M., Schwartz L. B., Durham S. R., Jeffery P. K., Kay A. B. Eosinophils, T-lymphocytes, mast cells, neutrophils, and macrophages in bronchial biopsy specimens from atopic subjects with asthma: comparison with biopsy specimens from atopic subjects without asthma and normal control subjects and relationship to bronchial hyperresponsiveness. J Allergy Clin Immunol. 1991 Oct;88(4):661–674. doi: 10.1016/0091-6749(91)90160-p. [DOI] [PubMed] [Google Scholar]
  9. Brandtzaeg P., Jahnsen F. L., Farstad I. N. Immune functions and immunopathology of the mucosa of the upper respiratory pathways. Acta Otolaryngol. 1996 Mar;116(2):149–159. doi: 10.3109/00016489609137812. [DOI] [PubMed] [Google Scholar]
  10. Brusasco V., Crimi E., Gianiorio P., Lantero S., Rossi G. A. Allergen-induced increase in airway responsiveness and inflammation in mild asthma. J Appl Physiol (1985) 1990 Dec;69(6):2209–2214. doi: 10.1152/jappl.1990.69.6.2209. [DOI] [PubMed] [Google Scholar]
  11. Brusasco V., Crimi E., Pellegrino R. Airway hyperresponsiveness in asthma: not just a matter of airway inflammation. Thorax. 1998 Nov;53(11):992–998. doi: 10.1136/thx.53.11.992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Carroll N., Cooke C., James A. The distribution of eosinophils and lymphocytes in the large and small airways of asthmatics. Eur Respir J. 1997 Feb;10(2):292–300. doi: 10.1183/09031936.97.10020292. [DOI] [PubMed] [Google Scholar]
  13. Cartier A., Thomson N. C., Frith P. A., Roberts R., Hargreave F. E. Allergen-induced increase in bronchial responsiveness to histamine: relationship to the late asthmatic response and change in airway caliber. J Allergy Clin Immunol. 1982 Sep;70(3):170–177. doi: 10.1016/0091-6749(82)90038-0. [DOI] [PubMed] [Google Scholar]
  14. Chung K. F. Leukotriene receptor antagonists and biosynthesis inhibitors: potential breakthrough in asthma therapy. Eur Respir J. 1995 Jul;8(7):1203–1213. doi: 10.1183/09031936.95.08071203. [DOI] [PubMed] [Google Scholar]
  15. Cockcroft D. W., Ruffin R. E., Dolovich J., Hargreave F. E. Allergen-induced increase in non-allergic bronchial reactivity. Clin Allergy. 1977 Nov;7(6):503–513. doi: 10.1111/j.1365-2222.1977.tb01481.x. [DOI] [PubMed] [Google Scholar]
  16. Corrigan C. J., Hamid Q., North J., Barkans J., Moqbel R., Durham S., Gemou-Engesaeth V., Kay A. B. Peripheral blood CD4 but not CD8 t-lymphocytes in patients with exacerbation of asthma transcribe and translate messenger RNA encoding cytokines which prolong eosinophil survival in the context of a Th2-type pattern: effect of glucocorticoid therapy. Am J Respir Cell Mol Biol. 1995 May;12(5):567–578. doi: 10.1165/ajrcmb.12.5.7742019. [DOI] [PubMed] [Google Scholar]
  17. Crimi E., Spanevello A., Neri M., Ind P. W., Rossi G. A., Brusasco V. Dissociation between airway inflammation and airway hyperresponsiveness in allergic asthma. Am J Respir Crit Care Med. 1998 Jan;157(1):4–9. doi: 10.1164/ajrccm.157.1.9703002. [DOI] [PubMed] [Google Scholar]
  18. DUNNILL M. S. The pathology of asthma, with special reference to changes in the bronchial mucosa. J Clin Pathol. 1960 Jan;13:27–33. doi: 10.1136/jcp.13.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. De Monchy J. G., Kauffman H. F., Venge P., Koëter G. H., Jansen H. M., Sluiter H. J., De Vries K. Bronchoalveolar eosinophilia during allergen-induced late asthmatic reactions. Am Rev Respir Dis. 1985 Mar;131(3):373–376. doi: 10.1164/arrd.1985.131.3.373. [DOI] [PubMed] [Google Scholar]
  20. Djukanović R., Wilson J. W., Britten K. M., Wilson S. J., Walls A. F., Roche W. R., Howarth P. H., Holgate S. T. Quantitation of mast cells and eosinophils in the bronchial mucosa of symptomatic atopic asthmatics and healthy control subjects using immunohistochemistry. Am Rev Respir Dis. 1990 Oct;142(4):863–871. doi: 10.1164/ajrccm/142.4.863. [DOI] [PubMed] [Google Scholar]
  21. Dunnill M. S., Massarella G. R., Anderson J. A. A comparison of the quantitative anatomy of the bronchi in normal subjects, in status asthmaticus, in chronic bronchitis, and in emphysema. Thorax. 1969 Mar;24(2):176–179. doi: 10.1136/thx.24.2.176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Durham S. R., Kay A. B. Eosinophils, bronchial hyperreactivity and late-phase asthmatic reactions. Clin Allergy. 1985 Sep;15(5):411–418. doi: 10.1111/j.1365-2222.1985.tb02290.x. [DOI] [PubMed] [Google Scholar]
  23. Erjefält J. S., Sundler F., Persson C. G. Eosinophils, neutrophils, and venular gaps in the airway mucosa at epithelial removal-restitution. Am J Respir Crit Care Med. 1996 May;153(5):1666–1674. doi: 10.1164/ajrccm.153.5.8630618. [DOI] [PubMed] [Google Scholar]
  24. Fahy J. V., Kim K. W., Liu J., Boushey H. A. Prominent neutrophilic inflammation in sputum from subjects with asthma exacerbation. J Allergy Clin Immunol. 1995 Apr;95(4):843–852. doi: 10.1016/s0091-6749(95)70128-1. [DOI] [PubMed] [Google Scholar]
  25. Frew A. J., Moqbel R., Azzawi M., Hartnell A., Barkans J., Jeffery P. K., Kay A. B., Scheper R. J., Varley J., Church M. K. T lymphocytes and eosinophils in allergen-induced late-phase asthmatic reactions in the guinea pig. Am Rev Respir Dis. 1990 Feb;141(2):407–413. doi: 10.1164/ajrccm/141.2.407. [DOI] [PubMed] [Google Scholar]
  26. Gleich G. J., Flavahan N. A., Fujisawa T., Vanhoutte P. M. The eosinophil as a mediator of damage to respiratory epithelium: a model for bronchial hyperreactivity. J Allergy Clin Immunol. 1988 May;81(5 Pt 1):776–781. doi: 10.1016/0091-6749(88)90931-1. [DOI] [PubMed] [Google Scholar]
  27. Haley K. J., Drazen J. M. Inflammation and airway function in asthma: what you see is not necessarily what you get. Am J Respir Crit Care Med. 1998 Jan;157(1):1–3. doi: 10.1164/ajrccm.157.1.ed-19. [DOI] [PubMed] [Google Scholar]
  28. Haley K. J., Sunday M. E., Wiggs B. R., Kozakewich H. P., Reilly J. J., Mentzer S. J., Sugarbaker D. J., Doerschuk C. M., Drazen J. M. Inflammatory cell distribution within and along asthmatic airways. Am J Respir Crit Care Med. 1998 Aug;158(2):565–572. doi: 10.1164/ajrccm.158.2.9705036. [DOI] [PubMed] [Google Scholar]
  29. Hamid Q., Song Y., Kotsimbos T. C., Minshall E., Bai T. R., Hegele R. G., Hogg J. C. Inflammation of small airways in asthma. J Allergy Clin Immunol. 1997 Jul;100(1):44–51. doi: 10.1016/s0091-6749(97)70193-3. [DOI] [PubMed] [Google Scholar]
  30. Hammermann R., Hirschmann J., Hey C., Mössner J., Folkerts G., Nijkamp F. P., Wessler I., Racké K. Cationic proteins inhibit L-arginine uptake in rat alveolar macrophages and tracheal epithelial cells. Implications for nitric oxide synthesis. Am J Respir Cell Mol Biol. 1999 Aug;21(2):155–162. doi: 10.1165/ajrcmb.21.2.3574. [DOI] [PubMed] [Google Scholar]
  31. Iredale M. J., Wanklyn S. A., Phillips I. P., Krausz T., Ind P. W. Non-invasive assessment of bronchial inflammation in asthma: no correlation between eosinophilia of induced sputum and bronchial responsiveness to inhaled hypertonic saline. Clin Exp Allergy. 1994 Oct;24(10):940–945. doi: 10.1111/j.1365-2222.1994.tb02725.x. [DOI] [PubMed] [Google Scholar]
  32. Jacoby D. B., Gleich G. J., Fryer A. D. Human eosinophil major basic protein is an endogenous allosteric antagonist at the inhibitory muscarinic M2 receptor. J Clin Invest. 1993 Apr;91(4):1314–1318. doi: 10.1172/JCI116331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. James A. L., Paré P. D., Hogg J. C. The mechanics of airway narrowing in asthma. Am Rev Respir Dis. 1989 Jan;139(1):242–246. doi: 10.1164/ajrccm/139.1.242. [DOI] [PubMed] [Google Scholar]
  34. Jatakanon A., Uasuf C., Maziak W., Lim S., Chung K. F., Barnes P. J. Neutrophilic inflammation in severe persistent asthma. Am J Respir Crit Care Med. 1999 Nov;160(5 Pt 1):1532–1539. doi: 10.1164/ajrccm.160.5.9806170. [DOI] [PubMed] [Google Scholar]
  35. Jeffery P. K., Wardlaw A. J., Nelson F. C., Collins J. V., Kay A. B. Bronchial biopsies in asthma. An ultrastructural, quantitative study and correlation with hyperreactivity. Am Rev Respir Dis. 1989 Dec;140(6):1745–1753. doi: 10.1164/ajrccm/140.6.1745. [DOI] [PubMed] [Google Scholar]
  36. Kelly C., Ward C., Stenton C. S., Bird G., Hendrick D. J., Walters E. H. Number and activity of inflammatory cells in bronchoalveolar lavage fluid in asthma and their relation to airway responsiveness. Thorax. 1988 Sep;43(9):684–692. doi: 10.1136/thx.43.9.684. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Kidney J. C., Wong A. G., Efthimiadis A., Morris M. M., Sears M. R., Dolovich J., Hargreave F. E. Elevated B cells in sputum of asthmatics. Close correlation with eosinophils. Am J Respir Crit Care Med. 1996 Feb;153(2):540–544. doi: 10.1164/ajrccm.153.2.8564094. [DOI] [PubMed] [Google Scholar]
  38. Kirby J. G., Hargreave F. E., Gleich G. J., O'Byrne P. M. Bronchoalveolar cell profiles of asthmatic and nonasthmatic subjects. Am Rev Respir Dis. 1987 Aug;136(2):379–383. doi: 10.1164/ajrccm/136.2.379. [DOI] [PubMed] [Google Scholar]
  39. Kloprogge E., de Leeuw A. J., de Monchy J. G., Kauffman H. F. Cellular communication in leukotriene C4 production between eosinophils and neutrophils. Int Arch Allergy Appl Immunol. 1989;90(1):20–23. doi: 10.1159/000234994. [DOI] [PubMed] [Google Scholar]
  40. Lambert R. K., Paré P. D. Lung parenchymal shear modulus, airway wall remodeling, and bronchial hyperresponsiveness. J Appl Physiol (1985) 1997 Jul;83(1):140–147. doi: 10.1152/jappl.1997.83.1.140. [DOI] [PubMed] [Google Scholar]
  41. Lamblin C., Gosset P., Tillie-Leblond I., Saulnier F., Marquette C. H., Wallaert B., Tonnel A. B. Bronchial neutrophilia in patients with noninfectious status asthmaticus. Am J Respir Crit Care Med. 1998 Feb;157(2):394–402. doi: 10.1164/ajrccm.157.2.97-02099. [DOI] [PubMed] [Google Scholar]
  42. Lapa e Silva J. R., Bachelet C. M., Pretolani M., Baker D., Scheper R. J., Vargaftig B. B. Immunopathologic alterations in the bronchi of immunized guinea pigs. Am J Respir Cell Mol Biol. 1993 Jul;9(1):44–53. doi: 10.1165/ajrcmb/9.1.44. [DOI] [PubMed] [Google Scholar]
  43. Meltzer S., Goldberg B., Lad P., Easton J. Superoxide generation and its modulation by adenosine in the neutrophils of subjects with asthma. J Allergy Clin Immunol. 1989 May;83(5):960–966. doi: 10.1016/0091-6749(89)90112-7. [DOI] [PubMed] [Google Scholar]
  44. Metzger W. J., Zavala D., Richerson H. B., Moseley P., Iwamota P., Monick M., Sjoerdsma K., Hunninghake G. W. Local allergen challenge and bronchoalveolar lavage of allergic asthmatic lungs. Description of the model and local airway inflammation. Am Rev Respir Dis. 1987 Feb;135(2):433–440. doi: 10.1164/arrd.1987.135.2.433. [DOI] [PubMed] [Google Scholar]
  45. Meurs H., Schuurman F. E., Duyvendak M., Zaagsma J. Deficiency of nitric oxide in polycation-induced airway hyperreactivity. Br J Pharmacol. 1999 Feb;126(3):559–562. doi: 10.1038/sj.bjp.0702372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Minette P. A., Lammers J. W., Dixon C. M., McCusker M. T., Barnes P. J. A muscarinic agonist inhibits reflex bronchoconstriction in normal but not in asthmatic subjects. J Appl Physiol (1985) 1989 Dec;67(6):2461–2465. doi: 10.1152/jappl.1989.67.6.2461. [DOI] [PubMed] [Google Scholar]
  47. Ollerenshaw S. L., Woolcock A. J. Characteristics of the inflammation in biopsies from large airways of subjects with asthma and subjects with chronic airflow limitation. Am Rev Respir Dis. 1992 Apr;145(4 Pt 1):922–927. doi: 10.1164/ajrccm/145.4_Pt_1.922. [DOI] [PubMed] [Google Scholar]
  48. Pin I., Radford S., Kolendowicz R., Jennings B., Denburg J. A., Hargreave F. E., Dolovich J. Airway inflammation in symptomatic and asymptomatic children with methacholine hyperresponsiveness. Eur Respir J. 1993 Oct;6(9):1249–1256. [PubMed] [Google Scholar]
  49. Pizzichini E., Pizzichini M. M., Efthimiadis A., Evans S., Morris M. M., Squillace D., Gleich G. J., Dolovich J., Hargreave F. E. Indices of airway inflammation in induced sputum: reproducibility and validity of cell and fluid-phase measurements. Am J Respir Crit Care Med. 1996 Aug;154(2 Pt 1):308–317. doi: 10.1164/ajrccm.154.2.8756799. [DOI] [PubMed] [Google Scholar]
  50. Ricciardolo F. L., Geppetti P., Mistretta A., Nadel J. A., Sapienza M. A., Bellofiore S., Di Maria G. U. Randomised double-blind placebo-controlled study of the effect of inhibition of nitric oxide synthesis in bradykinin-induced asthma. Lancet. 1996 Aug 10;348(9024):374–377. doi: 10.1016/s0140-6736(96)04450-9. [DOI] [PubMed] [Google Scholar]
  51. Sadeghi-Hashjin G., Folkerts G., Henricks P. A., Verheyen A. K., van der Linde H. J., van Ark I., Coene A., Nijkamp F. P. Peroxynitrite induces airway hyperresponsiveness in guinea pigs in vitro and in vivo. Am J Respir Crit Care Med. 1996 May;153(5):1697–1701. doi: 10.1164/ajrccm.153.5.8630623. [DOI] [PubMed] [Google Scholar]
  52. Saetta M., Di Stefano A., Rosina C., Thiene G., Fabbri L. M. Quantitative structural analysis of peripheral airways and arteries in sudden fatal asthma. Am Rev Respir Dis. 1991 Jan;143(1):138–143. doi: 10.1164/ajrccm/143.1.138. [DOI] [PubMed] [Google Scholar]
  53. Santing R. E., Hoekstra Y., Pasman Y., Zaagsma J., Meurs H. The importance of eosinophil activation for the development of allergen-induced bronchial hyperreactivity in conscious, unrestrained guinea-pigs. Clin Exp Allergy. 1994 Dec;24(12):1157–1163. doi: 10.1111/j.1365-2222.1994.tb03322.x. [DOI] [PubMed] [Google Scholar]
  54. Santing R. E., Meurs H., van der Mark T. W., Remie R., Oosterom W. C., Brouwer F., Zaagsma J. A novel method to assess airway function parameters in chronically instrumented, unrestrained guinea-pigs. Pulm Pharmacol. 1992 Dec;5(4):265–272. doi: 10.1016/0952-0600(92)90069-s. [DOI] [PubMed] [Google Scholar]
  55. Santing R. E., Olymulder C. G., Zaagsma J., Meurs H. Relationships among allergen-induced early and late phase airway obstructions, bronchial hyperreactivity, and inflammation in conscious, unrestrained guinea pigs. J Allergy Clin Immunol. 1994 Jun;93(6):1021–1030. doi: 10.1016/s0091-6749(94)70051-6. [DOI] [PubMed] [Google Scholar]
  56. Santing R. E., Pasman Y., Olymulder C. G., Roffel A. F., Meurs H., Zaagsma J. Contribution of a cholinergic reflex mechanism to allergen-induced bronchial hyperreactivity in permanently instrumented, unrestrained guinea-pigs. Br J Pharmacol. 1995 Jan;114(2):414–418. doi: 10.1111/j.1476-5381.1995.tb13242.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  57. Santing R. E., Schraa E. O., Wachters A., Olymulder C. G., Zaagsma J., Meurs H. Role of histamine in allergen-induced asthmatic reactions, bronchial hyperreactivity and inflammation in unrestrained guinea pigs. Eur J Pharmacol. 1994 Mar 11;254(1-2):49–57. doi: 10.1016/0014-2999(94)90369-7. [DOI] [PubMed] [Google Scholar]
  58. Schuiling M., Zuidhof A. B., Zaagsma J., Meurs H. Role of tachykinin NK1 and NK2 receptors in allergen-induced early and late asthmatic reactions, airway hyperresponsiveness, and airway inflammation in conscious, unrestrained guinea pigs. Clin Exp Allergy. 1999 Jun;29 (Suppl 2):48–52. doi: 10.1046/j.1365-2222.1999.00008.x. [DOI] [PubMed] [Google Scholar]
  59. Shute J. K., Vrugt B., Lindley I. J., Holgate S. T., Bron A., Aalbers R., Djukanović R. Free and complexed interleukin-8 in blood and bronchial mucosa in asthma. Am J Respir Crit Care Med. 1997 Jun;155(6):1877–1883. doi: 10.1164/ajrccm.155.6.9196089. [DOI] [PubMed] [Google Scholar]
  60. Sur S., Crotty T. B., Kephart G. M., Hyma B. A., Colby T. V., Reed C. E., Hunt L. W., Gleich G. J. Sudden-onset fatal asthma. A distinct entity with few eosinophils and relatively more neutrophils in the airway submucosa? Am Rev Respir Dis. 1993 Sep;148(3):713–719. doi: 10.1164/ajrccm/148.3.713. [DOI] [PubMed] [Google Scholar]
  61. Synek M., Beasley R., Frew A. J., Goulding D., Holloway L., Lampe F. C., Roche W. R., Holgate S. T. Cellular infiltration of the airways in asthma of varying severity. Am J Respir Crit Care Med. 1996 Jul;154(1):224–230. doi: 10.1164/ajrccm.154.1.8680684. [DOI] [PubMed] [Google Scholar]
  62. Tan B. T., Ekelaar F., Luirink J., Rimmelzwaan G., De Jonge A. J., Scheper R. J. Production of monoclonal antibodies defining guinea pig T-cell surface markers and a strain 13 Ia-like antigen: the value of immunohistological screening. Hybridoma. 1985 Summer;4(2):115–124. doi: 10.1089/hyb.1985.4.115. [DOI] [PubMed] [Google Scholar]
  63. Underwood S., Foster M., Raeburn D., Bottoms S., Karlsson J. A. Time-course of antigen-induced airway inflammation in the guinea-pig and its relationship to airway hyperresponsiveness. Eur Respir J. 1995 Dec;8(12):2104–2113. doi: 10.1183/109031936.95.08122104. [DOI] [PubMed] [Google Scholar]
  64. Wardlaw A. J., Dunnette S., Gleich G. J., Collins J. V., Kay A. B. Eosinophils and mast cells in bronchoalveolar lavage in subjects with mild asthma. Relationship to bronchial hyperreactivity. Am Rev Respir Dis. 1988 Jan;137(1):62–69. doi: 10.1164/ajrccm/137.1.62. [DOI] [PubMed] [Google Scholar]
  65. Wenzel S. E., Szefler S. J., Leung D. Y., Sloan S. I., Rex M. D., Martin R. J. Bronchoscopic evaluation of severe asthma. Persistent inflammation associated with high dose glucocorticoids. Am J Respir Crit Care Med. 1997 Sep;156(3 Pt 1):737–743. doi: 10.1164/ajrccm.156.3.9610046. [DOI] [PubMed] [Google Scholar]
  66. Woolley K. L., Adelroth E., Woolley M. J., Ellis R., Jordana M., O'Byrne P. M. Granulocyte-macrophage colony-stimulating factor, eosinophils and eosinophil cationic protein in subjects with and without mild, stable, atopic asthma. Eur Respir J. 1994 Sep;7(9):1576–1584. doi: 10.1183/09031936.94.07091576. [DOI] [PubMed] [Google Scholar]
  67. de Boer J., Meurs H., Coers W., Koopal M., Bottone A. E., Visser A. C., Timens W., Zaagsma J. Deficiency of nitric oxide in allergen-induced airway hyperreactivity to contractile agonists after the early asthmatic reaction: an ex vivo study. Br J Pharmacol. 1996 Nov;119(6):1109–1116. doi: 10.1111/j.1476-5381.1996.tb16011.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  68. ten Berge R. E., Santing R. E., Hamstra J. J., Roffel A. F., Zaagsma J. Dysfunction of muscarinic M2 receptors after the early allergic reaction: possible contribution to bronchial hyperresponsiveness in allergic guinea-pigs. Br J Pharmacol. 1995 Feb;114(4):881–887. doi: 10.1111/j.1476-5381.1995.tb13286.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  69. van Amsterdam R. G., Brouwer F., Zaagsma J. Analysis of the beta-adrenoceptor mediated inhibition of IgG1 and IgE dependent guinea-pig anaphylactic tracheal smooth muscle contraction. Agents Actions. 1989 Jan;26(1-2):48–51. doi: 10.1007/BF02126559. [DOI] [PubMed] [Google Scholar]

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