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. 2001 Aug;10(4):223–227. doi: 10.1080/09629350123332

Myeloperoxidase and adenosine-deaminase levels in the pleural fluid leakage induced by carrageenan in the mouse model of pleurisy.

T S Fröde 1, Y S Medeiros 1
PMCID: PMC1781707  PMID: 11577999

Abstract

BACKGROUND: Although myeloperoxidase (MPO) and adenosine-deaminase (ADA) levels are markers of activated leukocytes, both enzymes have not been currently addressed in inflammation models. AIMS: This study evaluates whether the concentrations of these enzymes are significantly correlated with the content of leukocytes in a pleurisy model. METHODS: The pleurisy was induced by carrageenan (1%) in mice, and the parameters analyzed 4 and 48 h after. RESULTS: After the induction of inflammation (4h), MPO and ADA levels peaked in parallel to neutrophils (p<0.01). Regarding the second phase of pleurisy (48 h), the highest concentrations of ADA were detected in parallel to the highest levels of mononuclears (p<0.01). At this time, MPO levels and neutrophils remained elevated, although at lower levels than those found at 4 h. A significant positive correlation was found among neutrophiLs and MPO, and mononuclears and ADA (p<0.01). CONCLUSIONS: These findings support the evidence that both enzymes are markers of the inflammatory process, and provide new tools for a better understanding of the immunoregulatory pathways that occur in inflammation.

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Selected References

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  1. Abd-Elfattah A. S., Messier R. H., Jr, Domkowski P. W., Jones J. L., Aly H. M., Crescenzo D. G., Wallace R. B., Hopkins R. A. Inhibition of adenosine deaminase and nucleoside transport. Utility in a model of homograft cardiac valve preimplantation processing. J Thorac Cardiovasc Surg. 1993 Jun;105(6):1095–1105. [PubMed] [Google Scholar]
  2. Bainton D. F. Distinct granule populations in human neutrophils and lysosomal organelles identified by immuno-electron microscopy. J Immunol Methods. 1999 Dec 17;232(1-2):153–168. doi: 10.1016/s0022-1759(99)00173-8. [DOI] [PubMed] [Google Scholar]
  3. Blackburn M. R., Datta S. K., Kellems R. E. Adenosine deaminase-deficient mice generated using a two-stage genetic engineering strategy exhibit a combined immunodeficiency. J Biol Chem. 1998 Feb 27;273(9):5093–5100. doi: 10.1074/jbc.273.9.5093. [DOI] [PubMed] [Google Scholar]
  4. Blackburn M. R., Volmer J. B., Thrasher J. L., Zhong H., Crosby J. R., Lee J. J., Kellems R. E. Metabolic consequences of adenosine deaminase deficiency in mice are associated with defects in alveogenesis, pulmonary inflammation, and airway obstruction. J Exp Med. 2000 Jul 17;192(2):159–170. doi: 10.1084/jem.192.2.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carr A. C., Myzak M. C., Stocker R., McCall M. R., Frei B. Myeloperoxidase binds to low-density lipoprotein: potential implications for atherosclerosis. FEBS Lett. 2000 Dec 29;487(2):176–180. doi: 10.1016/s0014-5793(00)02227-4. [DOI] [PubMed] [Google Scholar]
  6. Erel O., Kocyigit A., Gurel M. S., Bulut V., Seyrek A., Ozdemir Y. Adenosine deaminase activities in sera, lymphocytes and granulocytes in patients with cutaneous leishmaniasis. Mem Inst Oswaldo Cruz. 1998 Jul-Aug;93(4):491–494. doi: 10.1590/s0074-02761998000400014. [DOI] [PubMed] [Google Scholar]
  7. Gakis C., Calia G., Naitana A., Pirino D., Serru G. Serum adenosine deaminase activity in HIV positive subjects. A hypothesis on the significance of ADA2. Panminerva Med. 1989 Jul-Sep;31(3):107–113. [PubMed] [Google Scholar]
  8. Grooteman M. P., van Tellingen A., van Houte A. J., Bos J. C., Schoorl M., van Limbeek J., Nubé M. J. Hemodialysis-induced degranulation of polymorphonuclear cells: no correlation between membrane markers and degranulation products. Nephron. 2000 Jul;85(3):267–274. doi: 10.1159/000045671. [DOI] [PubMed] [Google Scholar]
  9. Herrera C., Casadó V., Ciruela F., Schofield P., Mallol J., Lluis C., Franco R. Adenosine A2B receptors behave as an alternative anchoring protein for cell surface adenosine deaminase in lymphocytes and cultured cells. Mol Pharmacol. 2001 Jan;59(1):127–134. [PubMed] [Google Scholar]
  10. Klebanoff S. J. Myeloperoxidase. Proc Assoc Am Physicians. 1999 Sep-Oct;111(5):383–389. doi: 10.1111/paa.1999.111.5.383. [DOI] [PubMed] [Google Scholar]
  11. Knoring B. E., Titarenko O. T., Sakharova I. Ia, Loginova G. P., D'iakova M. E., Perova T. L., Simbirtsev A. S., Pigareva N. V. Vzaimosviaz' produktsii tsitokinov i aktivnosti adenozindezaminazy pri tuberkuleze legkikh. Probl Tuberk. 2000;(3):38–41. [PubMed] [Google Scholar]
  12. Mishra O. P., Gupta B. L., Ali Z., Nath G., Chandra L. Adenosine deaminase activity in typhoid fever. Indian Pediatr. 1994 Nov;31(11):1379–1384. [PubMed] [Google Scholar]
  13. Rao T. S., Currie J. L., Shaffer A. F., Isakson P. C. Comparative evaluation of arachidonic acid (AA)- and tetradecanoylphorbol acetate (TPA)-induced dermal inflammation. Inflammation. 1993 Dec;17(6):723–741. doi: 10.1007/BF00920477. [DOI] [PubMed] [Google Scholar]
  14. Saleh T. S., Calixto J. B., Medeiros Y. S. Anti-inflammatory effects of theophylline, cromolyn and salbutamol in a murine model of pleurisy. Br J Pharmacol. 1996 Jun;118(3):811–819. doi: 10.1111/j.1476-5381.1996.tb15472.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Saleh T. S., Calixto J. B., Medeiros Y. S. Effects of anti-inflammatory drugs upon nitrate and myeloperoxidase levels in the mouse pleurisy induced by carrageenan. Peptides. 1999;20(8):949–956. doi: 10.1016/s0196-9781(99)00086-8. [DOI] [PubMed] [Google Scholar]
  16. Schmekel B., Seveus L., Xu S. Y., Venge P. Human neutrophil lipocalin (HNL) and myeloperoxidase (MPO). Studies of lung lavage fluid and lung tissue. Respir Med. 2000 Jun;94(6):564–568. doi: 10.1053/rmed.2000.0776. [DOI] [PubMed] [Google Scholar]
  17. Shende R. K., Bardapurker J., Patil V., Gaikwad A. Adenosine deaminase activity in leprosy. Indian J Lepr. 1993 Apr-Jun;65(2):201–205. [PubMed] [Google Scholar]
  18. Winterbourn C. C., Vissers M. C., Kettle A. J. Myeloperoxidase. Curr Opin Hematol. 2000 Jan;7(1):53–58. doi: 10.1097/00062752-200001000-00010. [DOI] [PubMed] [Google Scholar]
  19. Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain. 1983 Jun;16(2):109–110. doi: 10.1016/0304-3959(83)90201-4. [DOI] [PubMed] [Google Scholar]

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