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. 2001 Oct;10(5):269–272. doi: 10.1080/09629350120093740

Secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa.

M Nyström 1, U P Westin 1, C Linder 1, K Ohlsson 1
PMCID: PMC1781715  PMID: 11759111

Abstract

Secretory leukocyte protease inhibitor (SLPI) is a well-known protease inhibitor. Its function is thought to be protease/protease-inhibitor balance. Free proteolytic activity, mainly pancreatic elastase, anionic trypsin and granulocytic elastase, has been demonstrated in faecal extracts from patients with ulcerative colitis. We wanted to verify that SLPI is actually secreted from normal human colonic mucosa. Also, we wanted to ascertain whether studies of SLPI secretion based on punch biopsies were dependent on biopsy area or on biopsy circumference. Normal colonic mucosa was sampled during surgery for colonic cancer. A total of 36 samples from four patients were used. Mucosa preparation was carried out using a punch biopsy technique, and samples of 3, 4 and 6 mm diameter were used. All media contained SLPI at varying concentrations. When expressed in terms of the sample area, the secretion per millimetre-squared seemed to decrease with increasing area. When calculated as secretion per circumference, secretion seemed to be constant. In conclusion, SLPI was secreted from normal human colonic mucosa. The SLPI secretion seemed dependent on the circumference of the biopsy rather than on the area of the biopsy.

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Selected References

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  1. Bergenfeldt M., Nyström M., Bohe M., Lindström C., Polling A., Ohlsson K. Localization of immunoreactive secretory leukocyte protease inhibitor (SLPI) in intestinal mucosa. J Gastroenterol. 1996 Feb;31(1):18–23. doi: 10.1007/BF01211182. [DOI] [PubMed] [Google Scholar]
  2. Bohe M., Borgström A., Genell S., Ohlsson K. Determination of immunoreactive trypsin, pancreatic elastase and chymotrypsin in extracts of human feces and ileostomy drainage. Digestion. 1983;27(1):8–15. doi: 10.1159/000198913. [DOI] [PubMed] [Google Scholar]
  3. Bohe M. Pancreatic and granulocytic endoproteases in faecal extracts from patients with active ulcerative colitis. Scand J Gastroenterol. 1987 Jan;22(1):59–64. doi: 10.3109/00365528708991857. [DOI] [PubMed] [Google Scholar]
  4. Hiemstra P. S., Maassen R. J., Stolk J., Heinzel-Wieland R., Steffens G. J., Dijkman J. H. Antibacterial activity of antileukoprotease. Infect Immun. 1996 Nov;64(11):4520–4524. doi: 10.1128/iai.64.11.4520-4524.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hochstrasser K., Reichert R., Schwarz S., Werle E. isolierung und Charakterisierung eines Proteaseninhibitors aus menschlichen Bronchialsekret. Hoppe Seylers Z Physiol Chem. 1972 Feb;353(2):221–226. [PubMed] [Google Scholar]
  6. Håkansson H. O., Ohlsson K. Influence of plasma proteinase inhibitors and the secretory leucocyte proteinase inhibitor on pancreatic elastase-induced degradation of some plasma proteins. Gastroenterol Jpn. 1992 Oct;27(5):652–656. doi: 10.1007/BF02774981. [DOI] [PubMed] [Google Scholar]
  7. Kida K., Mizuuchi T., Takeyama K., Hiratsuka T., Jinno S., Hosoda K., Imaizumi A., Suzuki Y. Serum secretory leukoprotease inhibitor levels to diagnose pneumonia in the elderly. Am Rev Respir Dis. 1992 Dec;146(6):1426–1429. doi: 10.1164/ajrccm/146.6.1426. [DOI] [PubMed] [Google Scholar]
  8. Lee C. H., Igarashi Y., Hohman R. J., Kaulbach H., White M. V., Kaliner M. A. Distribution of secretory leukoprotease inhibitor in the human nasal airway. Am Rev Respir Dis. 1993 Mar;147(3):710–716. doi: 10.1164/ajrccm/147.3.710. [DOI] [PubMed] [Google Scholar]
  9. Masuda K., Kamimura T., Watanabe K., Suga T., Kanesaki M., Takeuchi A., Imaizumi A., Suzuki Y. Pharmacological activity of the C-terminal and N-terminal domains of secretory leukoprotease inhibitor in vitro. Br J Pharmacol. 1995 Jul;115(6):883–888. doi: 10.1111/j.1476-5381.1995.tb15892.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McNeely T. B., Dealy M., Dripps D. J., Orenstein J. M., Eisenberg S. P., Wahl S. M. Secretory leukocyte protease inhibitor: a human saliva protein exhibiting anti-human immunodeficiency virus 1 activity in vitro. J Clin Invest. 1995 Jul;96(1):456–464. doi: 10.1172/JCI118056. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McNeely T. B., Shugars D. C., Rosendahl M., Tucker C., Eisenberg S. P., Wahl S. M. Inhibition of human immunodeficiency virus type 1 infectivity by secretory leukocyte protease inhibitor occurs prior to viral reverse transcription. Blood. 1997 Aug 1;90(3):1141–1149. [PubMed] [Google Scholar]
  12. Mooren H. W., Kramps J. A., Franken C., Meijer C. J., Dijkman J. A. Localisation of a low-molecular-weight bronchial protease inhibitor in the peripheral human lung. Thorax. 1983 Mar;38(3):180–183. doi: 10.1136/thx.38.3.180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nyström M., Bergenfeldt M., Ohlsson K. The elimination of secretory leukocyte protease inhibitor (SLPI) from the gastrointestinal tract in man. Scand J Clin Lab Invest. 1997 Apr;57(2):119–125. doi: 10.1080/00365519709056379. [DOI] [PubMed] [Google Scholar]
  14. Ohlsson K., Tegner H. Inhibition of elastase from granulocytes by the low molecular weight bronchial protease inhibitor. Scand J Clin Lab Invest. 1976 Sep;36(5):437–445. doi: 10.3109/00365517609054461. [DOI] [PubMed] [Google Scholar]
  15. Ohlsson M., Rosengren M., Tegner H., Ohlsson K. Quantification of granulocyte elastase inhibitors in human mixed saliva and in pure parotid secretion. Hoppe Seylers Z Physiol Chem. 1983 Sep;364(9):1323–1328. doi: 10.1515/bchm2.1983.364.2.1323. [DOI] [PubMed] [Google Scholar]
  16. Tegner H., Ohlsson K., Olsson I. The interactions between a low molecular weight protease inhibitor of bronchial mucus and chymotrypsin-like cationic proteins of granulocytes. Hoppe Seylers Z Physiol Chem. 1977 Apr;358(4):431–433. doi: 10.1515/bchm2.1977.358.1.431. [DOI] [PubMed] [Google Scholar]
  17. Thompson R. C., Ohlsson K. Isolation, properties, and complete amino acid sequence of human secretory leukocyte protease inhibitor, a potent inhibitor of leukocyte elastase. Proc Natl Acad Sci U S A. 1986 Sep;83(18):6692–6696. doi: 10.1073/pnas.83.18.6692. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wallner O., Fritz H. Characterization of an acid-stable proteinase inhibitor in human cervical mucus. Hoppe Seylers Z Physiol Chem. 1974 Jun;355(6):709–715. doi: 10.1515/bchm2.1974.355.1.709. [DOI] [PubMed] [Google Scholar]

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