Skip to main content
PMC home page
Mediators of Inflammation logoLink to Mediators of Inflammation
. 2000;9(2):93–99. doi: 10.1080/096293500411550

Human eosinophil-airway smooth muscle cell interactions.

J M Hughes 1, C A Arthur 1, S Baracho 1, S M Carlin 1, K M Hawker 1, P R Johnson 1, C L Armour 1
PMCID: PMC1781747  PMID: 10958382

Abstract

Eosinophils are present throughout the airway wall of asthmatics. The nature of the interaction between human airway smooth muscle cells (ASMC) and eosinophils was investigated in this study. We demonstrated, using light microscopy, that freshly isolated eosinophils from healthy donors rapidly attach to ASMC in vitro. Numbers of attached eosinophils were highest at 2 h, falling to 50% of maximum by 20 h. Eosinophil attachment at 2 h was reduced to 72% of control by anti-VCAM-1, and to 74% at 20 h by anti-ICAM-1. Pre-treatment of ASMC for 24h with TNF-alpha, 10 nM, significantly increased eosinophil adhesion to 149 and 157% of control after 2 and 20 h. These results provide evidence that eosinophil interactions with ASMC involve VCAM-1 and ICAM-1 and are modulated by TNF-alpha.

Full Text

The Full Text of this article is available as a PDF (296.3 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnes P. J. Pharmacology of airway smooth muscle. Am J Respir Crit Care Med. 1998 Nov;158(5 Pt 3):S123–S132. doi: 10.1164/ajrccm.158.supplement_2.13tac800. [DOI] [PubMed] [Google Scholar]
  2. Broide D. H., Lotz M., Cuomo A. J., Coburn D. A., Federman E. C., Wasserman S. I. Cytokines in symptomatic asthma airways. J Allergy Clin Immunol. 1992 May;89(5):958–967. doi: 10.1016/0091-6749(92)90218-q. [DOI] [PubMed] [Google Scholar]
  3. Burke-Gaffney A., Hellewell P. G. A CD18/ICAM-1-dependent pathway mediates eosinophil adhesion to human bronchial epithelial cells. Am J Respir Cell Mol Biol. 1998 Sep;19(3):408–418. doi: 10.1165/ajrcmb.19.3.3179. [DOI] [PubMed] [Google Scholar]
  4. Chihara J., Yamamoto T., Kayaba H., Kakazu T., Kurachi D., Yamamoto J., Iwasa S., Iida K., Urayama O., Kobayashi Y. Degranulation of eosinophils mediated by intercellular adhesion molecule-1 and its ligands is involved in adhesion molecule expression on endothelial cells-selective induction of VCAM-1. J Allergy Clin Immunol. 1999 May;103(5 Pt 2):S452–S456. doi: 10.1016/s0091-6749(99)70161-2. [DOI] [PubMed] [Google Scholar]
  5. De S., Zelazny E. T., Souhrada J. F., Souhrada M. IL-1 beta and IL-6 induce hyperplasia and hypertrophy of cultured guinea pig airway smooth muscle cells. J Appl Physiol (1985) 1995 Apr;78(4):1555–1563. doi: 10.1152/jappl.1995.78.4.1555. [DOI] [PubMed] [Google Scholar]
  6. Egan R. W., Umland S. P., Cuss F. M., Chapman R. W. Biology of interleukin-5 and its relevance to allergic disease. Allergy. 1996 Feb;51(2):71–81. doi: 10.1111/j.1398-9995.1996.tb04561.x. [DOI] [PubMed] [Google Scholar]
  7. Fattah D., Page K. R., Bezbaruah S., Priest R. C., Horgan C. M., Solari R. A rapid activation assay for human eosinophils based on adhesion to immobilized ICAM-1, VCAM-1 and IgG. Cytokine. 1996 Mar;8(3):248–259. doi: 10.1006/cyto.1996.0034. [DOI] [PubMed] [Google Scholar]
  8. Ghaffar O., Hamid Q., Renzi P. M., Allakhverdi Z., Molet S., Hogg J. C., Shore S. A., Luster A. D., Lamkhioued B. Constitutive and cytokine-stimulated expression of eotaxin by human airway smooth muscle cells. Am J Respir Crit Care Med. 1999 Jun;159(6):1933–1942. doi: 10.1164/ajrccm.159.6.9805039. [DOI] [PubMed] [Google Scholar]
  9. Godding V., Stark J. M., Sedgwick J. B., Busse W. W. Adhesion of activated eosinophils to respiratory epithelial cells is enhanced by tumor necrosis factor-alpha and interleukin-1 beta. Am J Respir Cell Mol Biol. 1995 Nov;13(5):555–562. doi: 10.1165/ajrcmb.13.5.7576691. [DOI] [PubMed] [Google Scholar]
  10. Hallahan A. R., Armour C. L., Black J. L. Products of neutrophils and eosinophils increase the responsiveness of human isolated bronchial tissue. Eur Respir J. 1990 May;3(5):554–558. [PubMed] [Google Scholar]
  11. Hallsworth M. P., Soh C. P., Twort C. H., Lee T. H., Hirst S. J. Cultured human airway smooth muscle cells stimulated by interleukin-1beta enhance eosinophil survival. Am J Respir Cell Mol Biol. 1998 Dec;19(6):910–919. doi: 10.1165/ajrcmb.19.6.3275. [DOI] [PubMed] [Google Scholar]
  12. Hansel T. T., De Vries I. J., Iff T., Rihs S., Wandzilak M., Betz S., Blaser K., Walker C. An improved immunomagnetic procedure for the isolation of highly purified human blood eosinophils. J Immunol Methods. 1991 Dec 15;145(1-2):105–110. doi: 10.1016/0022-1759(91)90315-7. [DOI] [PubMed] [Google Scholar]
  13. Hawker K. M., Johnson P. R., Hughes J. M., Black J. L. Interleukin-4 inhibits mitogen-induced proliferation of human airway smooth muscle cells in culture. Am J Physiol. 1998 Sep;275(3 Pt 1):L469–L477. doi: 10.1152/ajplung.1998.275.3.L469. [DOI] [PubMed] [Google Scholar]
  14. Higashimoto I., Chihara J., Kakazu T., Yamamoto T., Kurachi D., Nakajima S. Adhesion to fibronectin augments eosinophil radical oxygen products. Int Arch Allergy Immunol. 1995;108 (Suppl 1):48–49. doi: 10.1159/000237202. [DOI] [PubMed] [Google Scholar]
  15. Horie S., Gleich G. J., Kita H. Cytokines directly induce degranulation and superoxide production from human eosinophils. J Allergy Clin Immunol. 1996 Aug;98(2):371–381. doi: 10.1016/s0091-6749(96)70161-6. [DOI] [PubMed] [Google Scholar]
  16. John M., Hirst S. J., Jose P. J., Robichaud A., Berkman N., Witt C., Twort C. H., Barnes P. J., Chung K. F. Human airway smooth muscle cells express and release RANTES in response to T helper 1 cytokines: regulation by T helper 2 cytokines and corticosteroids. J Immunol. 1997 Feb 15;158(4):1841–1847. [PubMed] [Google Scholar]
  17. Johnson P. R., Armour C. L., Carey D., Black J. L. Heparin and PGE2 inhibit DNA synthesis in human airway smooth muscle cells in culture. Am J Physiol. 1995 Oct;269(4 Pt 1):L514–L519. doi: 10.1152/ajplung.1995.269.4.L514. [DOI] [PubMed] [Google Scholar]
  18. Johnson P. R., Black J. L., Armour C. L. Platelet-activating factor-induced contraction of human isolated bronchus. Eur Respir J. 1992 Sep;5(8):970–974. [PubMed] [Google Scholar]
  19. Kita H., Horie S., Gleich G. J. Extracellular matrix proteins attenuate activation and degranulation of stimulated eosinophils. J Immunol. 1996 Feb 1;156(3):1174–1181. [PubMed] [Google Scholar]
  20. Lazaar A. L., Albelda S. M., Pilewski J. M., Brennan B., Puré E., Panettieri R. A., Jr T lymphocytes adhere to airway smooth muscle cells via integrins and CD44 and induce smooth muscle cell DNA synthesis. J Exp Med. 1994 Sep 1;180(3):807–816. doi: 10.1084/jem.180.3.807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Luttmann W., Matthiesen T., Matthys H., Virchow J. C., Jr Synergistic effects of interleukin-4 or interleukin-13 and tumor necrosis factor-alpha on eosinophil activation in vitro. Am J Respir Cell Mol Biol. 1999 Mar;20(3):474–480. doi: 10.1165/ajrcmb.20.3.3326. [DOI] [PubMed] [Google Scholar]
  22. Matsumoto K., Appiah-Pippim J., Schleimer R. P., Bickel C. A., Beck L. A., Bochner B. S. CD44 and CD69 represent different types of cell-surface activation markers for human eosinophils. Am J Respir Cell Mol Biol. 1998 Jun;18(6):860–866. doi: 10.1165/ajrcmb.18.6.3159. [DOI] [PubMed] [Google Scholar]
  23. Nagata M., Sedgwick J. B., Vrtis R., Busse W. W. Endothelial cells upregulate eosinophil superoxide generation via VCAM-1 expression. Clin Exp Allergy. 1999 Apr;29(4):550–561. doi: 10.1046/j.1365-2222.1999.00506.x. [DOI] [PubMed] [Google Scholar]
  24. Pang L., Knox A. J. Effect of interleukin-1 beta, tumour necrosis factor-alpha and interferon-gamma on the induction of cyclo-oxygenase-2 in cultured human airway smooth muscle cells. Br J Pharmacol. 1997 Jun;121(3):579–587. doi: 10.1038/sj.bjp.0701152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Platanias L. C., Vogelzang N. J. Interleukin-1: biology, pathophysiology, and clinical prospects. Am J Med. 1990 Nov;89(5):621–629. doi: 10.1016/0002-9343(90)90181-c. [DOI] [PubMed] [Google Scholar]
  26. Roubin R., Elsas P. P., Fiers W., Dessein A. J. Recombinant human tumour necrosis factor (rTNF)2 enhances leukotriene biosynthesis in neutrophils and eosinophils stimulated with the Ca2+ ionophore A23187. Clin Exp Immunol. 1987 Nov;70(2):484–490. [PMC free article] [PubMed] [Google Scholar]
  27. Sato M., Takizawa H., Kohyama T., Ohtoshi T., Takafuji S., Kawasaki S., Tohma S., Ishii A., Shoji S., Ito K. Eosinophil adhesion to human bronchial epithelial cells: regulation by cytokines. Int Arch Allergy Immunol. 1997 May-Jul;113(1-3):203–205. doi: 10.1159/000237547. [DOI] [PubMed] [Google Scholar]
  28. Seminario M. C., Gleich G. J. The role of eosinophils in the pathogenesis of asthma. Curr Opin Immunol. 1994 Dec;6(6):860–864. doi: 10.1016/0952-7915(94)90005-1. [DOI] [PubMed] [Google Scholar]
  29. Shah A., Church M. K., Holgate S. T. Tumour necrosis factor alpha: a potential mediator of asthma. Clin Exp Allergy. 1995 Nov;25(11):1038–1044. doi: 10.1111/j.1365-2222.1995.tb03249.x. [DOI] [PubMed] [Google Scholar]
  30. Stewart A. G., Tomlinson P. R., Fernandes D. J., Wilson J. W., Harris T. Tumor necrosis factor alpha modulates mitogenic responses of human cultured airway smooth muscle. Am J Respir Cell Mol Biol. 1995 Jan;12(1):110–119. doi: 10.1165/ajrcmb.12.1.7529028. [DOI] [PubMed] [Google Scholar]
  31. Viganò T., Habib A., Hernandez A., Bonazzi A., Boraschi D., Lebret M., Cassina E., Maclouf J., Sala A., Folco G. Cyclooxygenase-2 and synthesis of PGE2 in human bronchial smooth-muscle cells. Am J Respir Crit Care Med. 1997 Mar;155(3):864–868. doi: 10.1164/ajrccm.155.3.9117018. [DOI] [PubMed] [Google Scholar]
  32. Walsh G. M., Wardlaw A. J., Hartnell A., Sanderson C. J., Kay A. B. Interleukin-5 enhances the in vitro adhesion of human eosinophils, but not neutrophils, in a leucocyte integrin (CD11/18)-dependent manner. Int Arch Allergy Appl Immunol. 1991;94(1-4):174–178. doi: 10.1159/000235355. [DOI] [PubMed] [Google Scholar]
  33. Yoshida K., Suko M., Matsuzaki G., Sugiyama H., Okudaira H., Ito K. Effect of fibronectin on the production of leukotriene C4 by eosinophils. Int Arch Allergy Immunol. 1995;108 (Suppl 1):50–51. doi: 10.1159/000237203. [DOI] [PubMed] [Google Scholar]
  34. Zeck-Kapp G., Czech W., Kapp A. TNF alpha-induced activation of eosinophil oxidative metabolism and morphology--comparison with IL-5. Exp Dermatol. 1994 Aug;3(4):176–188. doi: 10.1111/j.1600-0625.1994.tb00275.x. [DOI] [PubMed] [Google Scholar]

Articles from Mediators of Inflammation are provided here courtesy of Wiley

RESOURCES