Abstract
BACKGROUND: Rheumatoid arthritis (RA) is an autoimmune disease with progressive activity. The RA remission was observed in women during pregnancy, but the mechanism responsible for remission is hypothetical only and concerns mechanisms of immune regulation such as lymphocyte subpopulations and interleukin production. AIMS: The lymphocyte subpopulations and interleukin production in vitro in a group of healthy non-pregnant women, healthy pregnant women and pregnant women suffering from RA may help towards a better understanding of regulation of the immune processes. METHODS: The investigations were performed in trimester III--2 days after delivery and 6 weeks after delivery. Peripheral blood lymphocytes were isolated on Gradisol gradient and analysed immediately or after having been cultured for 72 hours in RPMI medium supplemented with 10% FCS. The cultures were terminated after 72 h, supernatants stored at -72 degrees C for interleukin evaluation. The concentrations of IFN-gamma, IL-2, IL-6, IL-12, TNF-alpha and its soluble receptors R-I, R-II were estimated in non-stimulated and PHA (Sigma, 5 microg/ml) stimulated culture supernatants using ELISA Endogen kits according to the manufacturer's instructions. RESULTS: The general pattern of T cell subpopulation distribution was similar in all analysed groups. Decreased IFN-gamma, IL-12 and increased IL-6 production by lymphocytes after PHA stimulation was found in trimester III in pregnant women with RA as compared to healthy pregnant woman. CONCLUSION: The obtained results suggest that in pregnant women with RA the TH1 cell response predominates, contrary to healthy pregnant women with TH2 type functional response. These phenomena were not observed after delivery.
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- Arnett F. C., Edworthy S. M., Bloch D. A., McShane D. J., Fries J. F., Cooper N. S., Healey L. A., Kaplan S. R., Liang M. H., Luthra H. S. The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum. 1988 Mar;31(3):315–324. doi: 10.1002/art.1780310302. [DOI] [PubMed] [Google Scholar]
- Barrett J. H., Brennan P., Fiddler M., Silman A. J. Does rheumatoid arthritis remit during pregnancy and relapse postpartum? Results from a nationwide study in the United Kingdom performed prospectively from late pregnancy. Arthritis Rheum. 1999 Jun;42(6):1219–1227. doi: 10.1002/1529-0131(199906)42:6<1219::AID-ANR19>3.0.CO;2-G. [DOI] [PubMed] [Google Scholar]
- Butler D. M., Malfait A. M., Maini R. N., Brennan F. M., Feldmann M. Anti-IL-12 and anti-TNF antibodies synergistically suppress the progression of murine collagen-induced arthritis. Eur J Immunol. 1999 Jul;29(7):2205–2212. doi: 10.1002/(SICI)1521-4141(199907)29:07<2205::AID-IMMU2205>3.0.CO;2-Z. [DOI] [PubMed] [Google Scholar]
- Drakesmith H., Chain B., Beverley P. How can dendritic cells cause autoimmune disease? Immunol Today. 2000 May;21(5):214–217. doi: 10.1016/s0167-5699(00)01610-8. [DOI] [PubMed] [Google Scholar]
- Katsikis P. D., Chu C. Q., Brennan F. M., Maini R. N., Feldmann M. Immunoregulatory role of interleukin 10 in rheumatoid arthritis. J Exp Med. 1994 May 1;179(5):1517–1527. doi: 10.1084/jem.179.5.1517. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McRae B. L., Semnani R. T., Hayes M. P., van Seventer G. A. Type I IFNs inhibit human dendritic cell IL-12 production and Th1 cell development. J Immunol. 1998 May 1;160(9):4298–4304. [PubMed] [Google Scholar]
- Namekawa T., Snyder M. R., Yen J. H., Goehring B. E., Leibson P. J., Weyand C. M., Goronzy J. J. Killer cell activating receptors function as costimulatory molecules on CD4+CD28null T cells clonally expanded in rheumatoid arthritis. J Immunol. 2000 Jul 15;165(2):1138–1145. doi: 10.4049/jimmunol.165.2.1138. [DOI] [PubMed] [Google Scholar]
- Nelson J. L., Hughes K. A., Smith A. G., Nisperos B. B., Branchaud A. M., Hansen J. A. Remission of rheumatoid arthritis during pregnancy and maternal-fetal class II alloantigen disparity. Am J Reprod Immunol. 1992 Oct-Dec;28(3-4):226–227. doi: 10.1111/j.1600-0897.1992.tb00798.x. [DOI] [PubMed] [Google Scholar]
- Sacks G., Sargent I., Redman C. An innate view of human pregnancy. Immunol Today. 1999 Mar;20(3):114–118. doi: 10.1016/s0167-5699(98)01393-0. [DOI] [PubMed] [Google Scholar]
- Sasai M., Saeki Y., Ohshima S., Nishioka K., Mima T., Tanaka T., Katada Y., Yoshizaki K., Suemura M., Kishimoto T. Delayed onset and reduced severity of collagen-induced arthritis in interleukin-6-deficient mice. Arthritis Rheum. 1999 Aug;42(8):1635–1643. doi: 10.1002/1529-0131(199908)42:8<1635::AID-ANR11>3.0.CO;2-Q. [DOI] [PubMed] [Google Scholar]
- Schirmer M., Vallejo A. N., Weyand C. M., Goronzy J. J. Resistance to apoptosis and elevated expression of Bcl-2 in clonally expanded CD4+CD28- T cells from rheumatoid arthritis patients. J Immunol. 1998 Jul 15;161(2):1018–1025. [PubMed] [Google Scholar]
- Shinomiya M., Fazle Akbar S. M., Shinomiya H., Onji M. Transfer of dendritic cells (DC) ex vivo stimulated with interferon-gamma (IFN-gamma) down-modulates autoimmune diabetes in non-obese diabetic (NOD) mice. Clin Exp Immunol. 1999 Jul;117(1):38–43. doi: 10.1046/j.1365-2249.1999.00947.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spector T. D., Da Silva J. A. Pregnancy and rheumatoid arthritis: an overview. Am J Reprod Immunol. 1992 Oct-Dec;28(3-4):222–225. doi: 10.1111/j.1600-0897.1992.tb00797.x. [DOI] [PubMed] [Google Scholar]
- Wilder R. L. Hormones, pregnancy, and autoimmune diseases. Ann N Y Acad Sci. 1998 May 1;840:45–50. doi: 10.1111/j.1749-6632.1998.tb09547.x. [DOI] [PubMed] [Google Scholar]