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. 1999;8(4-5):189–197. doi: 10.1080/09629359990342

The mechanisms of action of interleukin-1 on rabbit intestinal epithelial cells.

F R Homaidan 1, L Zhao 1, I Chakroun 1, C A Martin 1, R Burakoff 1
PMCID: PMC1781803  PMID: 10704072

Abstract

Interleukin-1 (IL-1) is an inflammatory mediator that increases Cl- secretion in intestinal epithelial cells. To identify the signal transduction pathway(s) involved in IL-1's action, cells were treated with IL-1 and the levels of cyclooxygenase (COX) enzymes, prostaglandin E2 (PGE2) and phospholipase A2-activating protein (PLAP), and the activity of phospholipase A2 (PLA2) were measured. IL-1 caused concentration- and time-dependent increases in the levels of PLA2 activity, and/or in the levels of PLAP, COX-2 and PGE2. The IL-induced increase in PGE2 levels was biphasic, with the first peak due to the increase in PLAP levels, and the second peak due to the increase in COX-2 levels. This increase in PGE2 levels may provide a mechanism for acute and chronic inflammation in the intestine.

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Selected References

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