Skip to main content
PMC home page
Mediators of Inflammation logoLink to Mediators of Inflammation
. 1998;7(1):49–51. doi: 10.1080/09629359891388

In vitro activation of complement and contact system by lactic acidosis.

J Sonntag 1, M Emeis 1, E Strauss 1, M Obladen 1
PMCID: PMC1781817  PMID: 9839699

Abstract

The activation of complement and contact systems occurs in reperfusion injuries with initial tissue hypoxia, and lactic acidosis such as mycardial infarction and birth asphyxia. The aim of our experiment was the formal proof of activation by sole lactic acidosis. Lactic acid was added to blood and plasma samples from 10 healthy volunteers. C5a and factor XIIa were measured by EIA after incubation at 37 degrees C for 1 h. Both concentrations increased (P < 0.0001 by Friedman analysis) in blood and plasma samples with increasing amount of added lactic acid. Lactic acidosis can activate C5 from the complement system and factor XII from the contact system directly, even in the absence of cellular components.

Full Text

The Full Text of this article is available as a PDF (113.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chang T. M., Lister C. W. Assessment of blood substitutes: II. In-vitro complement activation of human plasma and blood for safety studies in research, development, industrial production and preclinical analysis. Artif Cells Blood Substit Immobil Biotechnol. 1994;22(2):171–180. doi: 10.3109/10731199409117412. [DOI] [PubMed] [Google Scholar]
  2. Fishelson Z., Horstmann R. D., Müller-Eberhard H. J. Regulation of the alternative pathway of complement by pH. J Immunol. 1987 May 15;138(10):3392–3395. [PubMed] [Google Scholar]
  3. Ford R. P., Esnouf M. P., Burgess A. I., Sarphie A. An enzyme-linked immunosorbent assay (ELISA) for the measurement of activated factor XII (Hageman factor) in human plasma. J Immunoassay. 1996 May;17(2):119–131. doi: 10.1080/01971529608005783. [DOI] [PubMed] [Google Scholar]
  4. Hammer C. H., Hänsch G., Gresham H. D., Shin M. L. Activation of the fifth and sixth components of the human complement system: C6-dependent cleavage of C5 in acid and the formation of a bimolecular lytic complex, C5b,6a. J Immunol. 1983 Aug;131(2):892–898. [PubMed] [Google Scholar]
  5. Hetland G., Moen O., Bergh K., Högäsen K., Hack C. E., Mollnes T. E., Fosse E. Both plasma- and leukocyte-associated C5a are essential for assessment of C5a generation in vivo. Ann Thorac Surg. 1997 Apr;63(4):1076–1080. doi: 10.1016/s0003-4975(96)01255-6. [DOI] [PubMed] [Google Scholar]
  6. Hill J., Lindsay T. F., Ortiz F., Yeh C. G., Hechtman H. B., Moore F. D., Jr Soluble complement receptor type 1 ameliorates the local and remote organ injury after intestinal ischemia-reperfusion in the rat. J Immunol. 1992 Sep 1;149(5):1723–1728. [PubMed] [Google Scholar]
  7. Horstick G., Heimann A., Götze O., Hafner G., Berg O., Böhmer P., Becker P., Darius H., Rupprecht H. J., Loos M. Intracoronary application of C1 esterase inhibitor improves cardiac function and reduces myocardial necrosis in an experimental model of ischemia and reperfusion. Circulation. 1997 Feb 4;95(3):701–708. doi: 10.1161/01.cir.95.3.701. [DOI] [PubMed] [Google Scholar]
  8. Klos A., Ihrig V., Messner M., Grabbe J., Bitter-Suermann D. Detection of native human complement components C3 and C5 and their primary activation peptides C3a and C5a (anaphylatoxic peptides) by ELISAs with monoclonal antibodies. J Immunol Methods. 1988 Jul 22;111(2):241–252. doi: 10.1016/0022-1759(88)90133-0. [DOI] [PubMed] [Google Scholar]
  9. Miyazawa K., Inoue K. Complement activation induced by human C-reactive protein in mildly acidic conditions. J Immunol. 1990 Jul 15;145(2):650–654. [PubMed] [Google Scholar]
  10. Mizock B. A. Lactic acidosis. Dis Mon. 1989 Apr;35(4):233–300. doi: 10.1016/0011-5029(89)90021-7. [DOI] [PubMed] [Google Scholar]
  11. Murdoch J., Hall R. Brain protection: physiological and pharmacological considerations. Part I: The physiology of brain injury. Can J Anaesth. 1990 Sep;37(6):663–671. doi: 10.1007/BF03006487. [DOI] [PubMed] [Google Scholar]
  12. Vandenberg R. J., Easterbrook-Smith S. B. Activation of the classical complement pathway by BioRex-70. Immunol Lett. 1987 Jan;14(2):155–158. doi: 10.1016/0165-2478(87)90095-2. [DOI] [PubMed] [Google Scholar]
  13. van der Kamp K. W., Hauch K. D., Feijen J., Horbett T. A. Contact activation during incubation of five different polyurethanes or glass in plasma. J Biomed Mater Res. 1995 Oct;29(10):1303–1306. doi: 10.1002/jbm.820291018. [DOI] [PubMed] [Google Scholar]

Articles from Mediators of Inflammation are provided here courtesy of Wiley

RESOURCES