Skip to main content
NCBI home page
Mediators of Inflammation logoLink to Mediators of Inflammation
. 1998;7(1):31–33. doi: 10.1080/09629359891351

Effects of lipoxygenase metabolites of arachidonic acid on the growth of human mononuclear marrow cells and marrow stromal cell cultures.

V Desplat 1, F Dupuis 1, F Trimoreau 1, C Dulery 1, V Praloran 1, Y Denizot 1
PMCID: PMC1781818  PMID: 9839696

Abstract

The effects of various lipoxygenase metabolites of arachidonic acid (AA) were investigated on the growth of freshly isolated human bone marrow mononuclear cells and marrow stromal cell cultures. LTB4, LXA4, LXB4, 12-HETE and 15-HETE (1 microM) decreased [3H]-thymidine incorporation on marrow stromal cell cultures without affecting cell number. Only 12-HETE showed a dose-response effect on [3H]-thymidine incorporation. While LTB4 (1 microM) decreased thymidine incorporation on marrow mononuclear cells, LTC4, LXA4, LXB4, 12-HETE and 15-HETE had no effect. The lipoxygenase inhibitor NDGA had no effect on both cell types suggesting no role of endogenous lipoxygenase metabolites on cell growth. These results suggest no important role of lipoxygenase metabolites of AA on the proliferation of human marrow mononuclear cells and marrow stromal cell cultures.

Full Text

The Full Text of this article is available as a PDF (134.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bazill G. W., Dexter T. M. Platelet activating factor antagonist L-652,731 inhibits thymidine transport. Biochem Pharmacol. 1989 Jan 15;38(2):373–374. doi: 10.1016/0006-2952(89)90052-x. [DOI] [PubMed] [Google Scholar]
  2. Claesson H. E., Dahlberg N., Gahrton G. Stimulation of human myelopoiesis by leukotriene B4. Biochem Biophys Res Commun. 1985 Sep 16;131(2):579–585. doi: 10.1016/0006-291x(85)91276-8. [DOI] [PubMed] [Google Scholar]
  3. Denizot Y., Dupuis F., Trimoreau F., Verger C., Allegraud A., Praloran V. PAF and haematopoiesis: IX. Platelet-activating factor increases DNA synthesis in human bone marrow cells. J Lipid Mediat Cell Signal. 1996 Dec;15(1):1–4. doi: 10.1016/s0929-7855(96)00447-6. [DOI] [PubMed] [Google Scholar]
  4. Denizot Y., Rougier F., Dupuis F., Verger C., Dulery C., Laskar M., Aldigier J. C., Praloran V. PAF and hematopoiesis. VIII. Biosynthesis and metabolism of PAF by human bone marrow stromal cells. Exp Hematol. 1996 Sep;24(11):1327–1332. [PubMed] [Google Scholar]
  5. Fixe P., Rougier F., Ostyn E., Gachard N., Faucher J. L., Praloran V., Denizot Y. Spontaneous and inducible production of macrophage colony-stimulating factor by human bone marrow stromal cells. Eur Cytokine Netw. 1997 Mar;8(1):91–95. [PubMed] [Google Scholar]
  6. Galmiche M. C., Koteliansky V. E., Brière J., Hervé P., Charbord P. Stromal cells from human long-term marrow cultures are mesenchymal cells that differentiate following a vascular smooth muscle differentiation pathway. Blood. 1993 Jul 1;82(1):66–76. [PubMed] [Google Scholar]
  7. Lee P. P., Ip M. M. Regulation of proliferation of rat mammary tumor cells by inhibitors of cyclooxygenase and lipoxygenase. Prostaglandins Leukot Essent Fatty Acids. 1992 Jan;45(1):21–31. doi: 10.1016/0952-3278(92)90098-4. [DOI] [PubMed] [Google Scholar]
  8. Mayani H., Guilbert L. J., Janowska-Wieczorek A. Biology of the hemopoietic microenvironment. Eur J Haematol. 1992 Nov;49(5):225–233. doi: 10.1111/j.1600-0609.1992.tb00053.x. [DOI] [PubMed] [Google Scholar]
  9. Miller A. M., Weiner R. S., Ziboh V. A. Evidence for the role of leukotrienes C4 and D4 as essential intermediates in CSF-stimulated human myeloid colony formation. Exp Hematol. 1986 Sep;14(8):760–765. [PubMed] [Google Scholar]
  10. Palmberg L., Lindgren J. A., Thyberg J., Claesson H. E. On the mechanism of induction of DNA synthesis in cultured arterial smooth muscle cells by leukotrienes. Possible role of prostaglandin endoperoxide synthase products and platelet-derived growth factor. J Cell Sci. 1991 Feb;98(Pt 2):141–149. doi: 10.1242/jcs.98.2.141. [DOI] [PubMed] [Google Scholar]
  11. Rougier F., Dupuis F., Cornu E., Dulery C., Praloran V., Denizot Y. Platelet-activating factor and antagonists modulate DNA synthesis in human bone marrow stromal cell cultures. J Lipid Mediat Cell Signal. 1997 Jul;16(3):147–153. doi: 10.1016/s0929-7855(97)00576-2. [DOI] [PubMed] [Google Scholar]
  12. Rougier F., Dupuis F., Denizot Y. Human bone marrow fibroblasts--an overview of their characterization, proliferation and inflammatory mediator production. Hematol Cell Ther. 1996 Jul;38(3):241–246. doi: 10.1007/s00282-996-0241-3. [DOI] [PubMed] [Google Scholar]
  13. Setty B. N., Graeber J. E., Stuart M. J. The mitogenic effect of 15- and 12-hydroxyeicosatetraenoic acid on endothelial cells may be mediated via diacylglycerol kinase inhibition. J Biol Chem. 1987 Dec 25;262(36):17613–17622. [PubMed] [Google Scholar]
  14. Smith D. L., Willis A. L., Mahmud I. Eicosanoid effects on cell proliferation in vitro: relevance to atherosclerosis. Prostaglandins Leukot Med. 1984 Oct;16(1):1–10. doi: 10.1016/0262-1746(84)90080-5. [DOI] [PubMed] [Google Scholar]
  15. Snyder D. S., Desforges J. F. Lipoxygenase metabolites of arachidonic acid modulate hematopoiesis. Blood. 1986 Jun;67(6):1675–1679. [PubMed] [Google Scholar]
  16. Stenke L., Laurén L., Reizenstein P., Lindgren J. A. Leukotriene production by fresh human bone marrow cells: evidence of altered lipoxygenase activity in chronic myelocytic leukemia. Exp Hematol. 1987 Feb;15(2):203–207. [PubMed] [Google Scholar]
  17. Stenke L., Mansour M., Edenius C., Reizenstein P., Lindgren J. A. Formation and proliferative effects of lipoxins in human bone marrow. Biochem Biophys Res Commun. 1991 Oct 15;180(1):255–261. doi: 10.1016/s0006-291x(05)81285-9. [DOI] [PubMed] [Google Scholar]
  18. Werner E. J., Walenga R. W., Dubowy R. L., Boone S., Stuart M. J. Inhibition of human malignant neuroblastoma cell DNA synthesis by lipoxygenase metabolites of arachidonic acid. Cancer Res. 1985 Feb;45(2):561–563. [PubMed] [Google Scholar]

Articles from Mediators of Inflammation are provided here courtesy of Wiley

RESOURCES