Skip to main content
NCBI home page
Mediators of Inflammation logoLink to Mediators of Inflammation
. 1998;7(1):13–18. doi: 10.1080/09629359891324

Rooperol tetraacetate decreases cytokine mRNA levels and binding capacity of transcription factors in U937 cells.

A Guzdek 1, H Rokita 1, J Cichy 1, A C Allison 1, A Koj 1
PMCID: PMC1781819  PMID: 9839693

Abstract

We have previously described inhibition of the synthesis of three acute-phase inflammatory cytokines in human and rat macrophages by acetate esters of rooperol, a dicatechol of plant origin. Analysing the mechanism of anticytokine activity of rooperol, we compared levels of TNFalpha, IL-1beta and IL-6 mRNAs in the human promonocytic U937 cell line pretreated with phorbol myristate acetate (PMA) and incubated with rooperol tetraacetate (RTA) alone or in combination with LPS (500 ng/ml). It was found that 10 microM RTA decreased the levels of cytokine mRNAs both in the presence and absence of LPS, suggesting pretranslational inhibition of cytokine synthesis. Electrophoretic mobility shift analysis (EMSA) showed that RTA may influence cytokine mRNA expression by decreasing the binding activity of transcription factors NF-kappaB and AP-1.

Full Text

The Full Text of this article is available as a PDF (464.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Angel P., Imagawa M., Chiu R., Stein B., Imbra R. J., Rahmsdorf H. J., Jonat C., Herrlich P., Karin M. Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor. Cell. 1987 Jun 19;49(6):729–739. doi: 10.1016/0092-8674(87)90611-8. [DOI] [PubMed] [Google Scholar]
  2. Barnes P. J., Liew F. Y. Nitric oxide and asthmatic inflammation. Immunol Today. 1995 Mar;16(3):128–130. doi: 10.1016/0167-5699(95)80128-6. [DOI] [PubMed] [Google Scholar]
  3. Bereta J., Bereta M., Allison A. C., Kruger P. B., Koj A. Inhibitory effect of di-catechol rooperol on VCAM-1 and iNOS expression in cytokine-stimulated endothelium. Life Sci. 1997;60(4-5):325–334. doi: 10.1016/s0024-3205(96)00633-9. [DOI] [PubMed] [Google Scholar]
  4. Breedveld F. Tenidap: a novel cytokine-modulating antirheumatic drug for the treatment of rheumatoid arthritis. Scand J Rheumatol Suppl. 1994;100:31–44. doi: 10.3109/03009749409095199. [DOI] [PubMed] [Google Scholar]
  5. Cavaillon J. M. Cytokines and macrophages. Biomed Pharmacother. 1994;48(10):445–453. doi: 10.1016/0753-3322(94)90005-1. [DOI] [PubMed] [Google Scholar]
  6. Chen C. Y., Shyu A. B. AU-rich elements: characterization and importance in mRNA degradation. Trends Biochem Sci. 1995 Nov;20(11):465–470. doi: 10.1016/s0968-0004(00)89102-1. [DOI] [PubMed] [Google Scholar]
  7. Cogswell J. P., Godlevski M. M., Wisely G. B., Clay W. C., Leesnitzer L. M., Ways J. P., Gray J. G. NF-kappa B regulates IL-1 beta transcription through a consensus NF-kappa B binding site and a nonconsensus CRE-like site. J Immunol. 1994 Jul 15;153(2):712–723. [PubMed] [Google Scholar]
  8. Dinarello C. A. Biologic basis for interleukin-1 in disease. Blood. 1996 Mar 15;87(6):2095–2147. [PubMed] [Google Scholar]
  9. Duyao M. P., Buckler A. J., Sonenshein G. E. Interaction of an NF-kappa B-like factor with a site upstream of the c-myc promoter. Proc Natl Acad Sci U S A. 1990 Jun;87(12):4727–4731. doi: 10.1073/pnas.87.12.4727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Guzdek A., Nizankowska E., Allison A. C., Kruger P. B., Koj A. Cytokine production in human and rat macrophages and dicatechol rooperol and esters. Biochem Pharmacol. 1996 Oct 11;52(7):991–998. doi: 10.1016/0006-2952(96)00386-3. [DOI] [PubMed] [Google Scholar]
  11. Kick G., Messer G., Goetz A., Plewig G., Kind P. Photodynamic therapy induces expression of interleukin 6 by activation of AP-1 but not NF-kappa B DNA binding. Cancer Res. 1995 Jun 1;55(11):2373–2379. [PubMed] [Google Scholar]
  12. Koj A. Initiation of acute phase response and synthesis of cytokines. Biochim Biophys Acta. 1996 Nov 15;1317(2):84–94. doi: 10.1016/s0925-4439(96)00048-8. [DOI] [PubMed] [Google Scholar]
  13. Laskin D. L., Pendino K. J. Macrophages and inflammatory mediators in tissue injury. Annu Rev Pharmacol Toxicol. 1995;35:655–677. doi: 10.1146/annurev.pa.35.040195.003255. [DOI] [PubMed] [Google Scholar]
  14. Lee J. C., Laydon J. T., McDonnell P. C., Gallagher T. F., Kumar S., Green D., McNulty D., Blumenthal M. J., Heys J. R., Landvatter S. W. A protein kinase involved in the regulation of inflammatory cytokine biosynthesis. Nature. 1994 Dec 22;372(6508):739–746. doi: 10.1038/372739a0. [DOI] [PubMed] [Google Scholar]
  15. Lee J. C., Young P. R. Role of CSB/p38/RK stress response kinase in LPS and cytokine signaling mechanisms. J Leukoc Biol. 1996 Feb;59(2):152–157. doi: 10.1002/jlb.59.2.152. [DOI] [PubMed] [Google Scholar]
  16. Lee S. W., Ilnicka M. Hydrogen peroxide primes promonocytic U937 cells to produce IL-1 beta. Ann N Y Acad Sci. 1993 Nov 30;696:399–401. doi: 10.1111/j.1749-6632.1993.tb17177.x. [DOI] [PubMed] [Google Scholar]
  17. Moore P. F., Larson D. L., Otterness I. G., Weissman A., Kadin S. B., Sweeney F. J., Eskra J. D., Nagahisa A., Sakakibara M., Carty T. J. Tenidap, a structurally novel drug for the treatment of arthritis: antiinflammatory and analgesic properties. Inflamm Res. 1996 Feb;45(2):54–61. doi: 10.1007/BF02265116. [DOI] [PubMed] [Google Scholar]
  18. Ounissi-Benkalha H., Pelletier J. P., Tardif G., Mineau F., Jolicoeur F. C., Ranger P., Martel-Pelletier J. In vitro effects of 2 antirheumatic drugs on the synthesis and expression of proinflammatory cytokines in synovial membranes from patients with rheumatoid arthritis. J Rheumatol. 1996 Jan;23(1):16–23. [PubMed] [Google Scholar]
  19. Rordorf-Adam C., Geiger T., Henn R., Arnold J., Solf R., Wiesenberg I., Ferrini P. G., Vosbeck K. CGP 47969A: a novel inhibitor of the synthesis of inflammatory cytokines. Agents Actions. 1994 Nov;43(1-2):53–59. doi: 10.1007/BF02005765. [DOI] [PubMed] [Google Scholar]
  20. Rose-John S., Dietrich A., Marks F. Molecular cloning of mouse protein kinase C (PKC) cDNA from Swiss 3T3 fibroblasts. Gene. 1988 Dec 30;74(2):465–471. doi: 10.1016/0378-1119(88)90179-5. [DOI] [PubMed] [Google Scholar]
  21. Schreck R., Albermann K., Baeuerle P. A. Nuclear factor kappa B: an oxidative stress-responsive transcription factor of eukaryotic cells (a review). Free Radic Res Commun. 1992;17(4):221–237. doi: 10.3109/10715769209079515. [DOI] [PubMed] [Google Scholar]
  22. Sehgal P. B., Wang L., Rayanade R., Pan H., Margulies L. Interleukin-6-type cytokines. Ann N Y Acad Sci. 1995 Jul 21;762:1–14. [PubMed] [Google Scholar]
  23. Suzuki Y. J., Mizuno M., Packer L. Signal transduction for nuclear factor-kappa B activation. Proposed location of antioxidant-inhibitable step. J Immunol. 1994 Dec 1;153(11):5008–5015. [PubMed] [Google Scholar]
  24. Takeshita S., Gage J. R., Kishimoto T., Vredevoe D. L., Martínez-Maza O. Differential regulation of IL-6 gene transcription and expression by IL-4 and IL-10 in human monocytic cell lines. J Immunol. 1996 Apr 1;156(7):2591–2598. [PubMed] [Google Scholar]
  25. Van der Merwe M. J., Jenkins K., Theron E., van der Walt B. J. Interaction of the di-catechols rooperol and nordihydroguaiaretic acid with oxidative systems in the human blood. A structure-activity relationship. Biochem Pharmacol. 1993 Jan 26;45(2):303–311. doi: 10.1016/0006-2952(93)90065-5. [DOI] [PubMed] [Google Scholar]
  26. Willeaume V., Kruys V., Mijatovic T., Huez G. Tumor necrosis factor-alpha production induced by viruses and by lipopolysaccharides in macrophages: similarities and differences. J Inflamm. 1995;46(1):1–12. [PubMed] [Google Scholar]
  27. Zhong W. W., Burke P. A., Hand A. T., Walsh M. J., Hughes L. A., Forse R. A. Regulation of cytokine mRNA expression in lipopolysaccharide-stimulated human macrophages. Arch Surg. 1993 Feb;128(2):158–164. doi: 10.1001/archsurg.1993.01420140035006. [DOI] [PubMed] [Google Scholar]

Articles from Mediators of Inflammation are provided here courtesy of Wiley

RESOURCES