Skip to main content
NCBI home page
Mediators of Inflammation logoLink to Mediators of Inflammation
. 1998;7(1):7–11. doi: 10.1080/09629359891315

The effect of interleukin-10 and transforming growth factor beta-1 on HLA-DR expression in colonic epithelial cells.

M J Zimmerman 1, G R Radford-Smith 1, D P Jewell 1
PMCID: PMC1781823  PMID: 9839692

Abstract

The aim of this study was to assess whether interleukin-10 (IL-10) and/or transforming growth factor beta-1 (TGFbeta1) downregulate HLA-DR expression using the HT29 cell line as a model of colonic epithelial cells. HLA-DR expression was induced in HT29 cells with gamma-interferon. The effects of IL-10 alone, TGFbeta1 alone, and IL-10 and TGFbeta1 in combination were studied. HLA-DR expression was assessed using flow cytometric analysis. Gamma-interferon induced HLA-DR expression in a dose-dependent fashion. In the absence of gamma-interferon, neither IL-10 nor TGFbeta1 induced HLA-DR expression. In isolation, neither IL-10 nor TGFbeta1 downregulated HLA-DR expression. When IL-10 and TGFbeta1 were added in combination, small (6-30%) statistically significant reductions in HLA-DR expression were seen. The biological significance is unclear.

Full Text

The Full Text of this article is available as a PDF (177.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Augeron C., Laboisse C. L. Emergence of permanently differentiated cell clones in a human colonic cancer cell line in culture after treatment with sodium butyrate. Cancer Res. 1984 Sep;44(9):3961–3969. [PubMed] [Google Scholar]
  2. Avery A., Paraskeva C., Hall P., Flanders K. C., Sporn M., Moorghen M. TGF-beta expression in the human colon: differential immunostaining along crypt epithelium. Br J Cancer. 1993 Jul;68(1):137–139. doi: 10.1038/bjc.1993.301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benoist C., Mathis D. Regulation of major histocompatibility complex class-II genes: X, Y and other letters of the alphabet. Annu Rev Immunol. 1990;8:681–715. doi: 10.1146/annurev.iy.08.040190.003341. [DOI] [PubMed] [Google Scholar]
  4. Bland P. MHC class II expression by the gut epithelium. Immunol Today. 1988 Jun;9(6):174–178. doi: 10.1016/0167-5699(88)91293-5. [DOI] [PubMed] [Google Scholar]
  5. Bogdan C., Nathan C. Modulation of macrophage function by transforming growth factor beta, interleukin-4, and interleukin-10. Ann N Y Acad Sci. 1993 Jun 23;685:713–739. doi: 10.1111/j.1749-6632.1993.tb35934.x. [DOI] [PubMed] [Google Scholar]
  6. Boorstein S. M., Elner S. G., Meyer R. F., Sugar A., Strieter R. M., Kunkel S. L., Elner V. M. Interleukin-10 inhibition of HLA-DR expression in human herpes stromal keratitis. Ophthalmology. 1994 Sep;101(9):1529–1535. doi: 10.1016/s0161-6420(94)31149-3. [DOI] [PubMed] [Google Scholar]
  7. Crotty B., Hoang P., Dalton H. R., Jewell D. P. Salicylates used in inflammatory bowel disease and colchicine impair interferon-gamma induced HLA-DR expression. Gut. 1992 Jan;33(1):59–64. doi: 10.1136/gut.33.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Daar A. S., Fuggle S. V., Fabre J. W., Ting A., Morris P. J. The detailed distribution of MHC Class II antigens in normal human organs. Transplantation. 1984 Sep;38(3):293–298. doi: 10.1097/00007890-198409000-00019. [DOI] [PubMed] [Google Scholar]
  9. Dalton H. R., Hoang P., Jewell D. P. Antigen induced suppression in peripheral blood and lamina propria mononuclear cells in inflammatory bowel disease. Gut. 1992 Mar;33(3):324–330. doi: 10.1136/gut.33.3.324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Darley R., Morris A., Passas J., Bateman W. Interactions between interferon gamma and retinoic acid with transforming growth factor beta in the induction of immune recognition molecules. Cancer Immunol Immunother. 1993 Jul;37(2):112–118. doi: 10.1007/BF01517043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Donnet-Hughes A., Schiffrin E. J., Huggett A. C. Expression of MHC antigens by intestinal epithelial cells. Effect of transforming growth factor-beta 2 (TGF-beta 2). Clin Exp Immunol. 1995 Feb;99(2):240–244. doi: 10.1111/j.1365-2249.1995.tb05539.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Fonti R., Latella G., Bises G., Magliocca F., Nobili F., Caprilli R., Sambuy Y. Human colonocytes in primary culture: a model to study epithelial growth, metabolism and differentiation. Int J Colorectal Dis. 1994 Apr;9(1):13–22. doi: 10.1007/BF00304294. [DOI] [PubMed] [Google Scholar]
  13. Frei K., Lins H., Schwerdel C., Fontana A. Antigen presentation in the central nervous system. The inhibitory effect of IL-10 on MHC class II expression and production of cytokines depends on the inducing signals and the type of cell analyzed. J Immunol. 1994 Mar 15;152(6):2720–2728. [PubMed] [Google Scholar]
  14. Isomaki P., Luukkainen R., Saario R., Toivanen P., Punnonen J. Interleukin-10 functions as an antiinflammatory cytokine in rheumatoid synovium. Arthritis Rheum. 1996 Mar;39(3):386–395. doi: 10.1002/art.1780390306. [DOI] [PubMed] [Google Scholar]
  15. Kühn R., Löhler J., Rennick D., Rajewsky K., Müller W. Interleukin-10-deficient mice develop chronic enterocolitis. Cell. 1993 Oct 22;75(2):263–274. doi: 10.1016/0092-8674(93)80068-p. [DOI] [PubMed] [Google Scholar]
  16. Lowes J. R., Radwan P., Priddle J. D., Jewell D. P. Characterisation and quantification of mucosal cytokine that induces epithelial histocompatibility locus antigen-DR expression in inflammatory bowel disease. Gut. 1992 Mar;33(3):315–319. doi: 10.1136/gut.33.3.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lundqvist C., Hammarström M. L., Athlin L., Hammarström S. Isolation of functionally active intraepithelial lymphocytes and enterocytes from human small and large intestine. J Immunol Methods. 1992 Aug 10;152(2):253–263. doi: 10.1016/0022-1759(92)90147-l. [DOI] [PubMed] [Google Scholar]
  18. Markowitz S., Wang J., Myeroff L., Parsons R., Sun L., Lutterbaugh J., Fan R. S., Zborowska E., Kinzler K. W., Vogelstein B. Inactivation of the type II TGF-beta receptor in colon cancer cells with microsatellite instability. Science. 1995 Jun 2;268(5215):1336–1338. doi: 10.1126/science.7761852. [DOI] [PubMed] [Google Scholar]
  19. Mayer L., Shlien R. Evidence for function of Ia molecules on gut epithelial cells in man. J Exp Med. 1987 Nov 1;166(5):1471–1483. doi: 10.1084/jem.166.5.1471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. McDonald G. B., Jewell D. P. Class II antigen (HLA-DR) expression by intestinal epithelial cells in inflammatory diseases of colon. J Clin Pathol. 1987 Mar;40(3):312–317. doi: 10.1136/jcp.40.3.312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mosmann T. R. Properties and functions of interleukin-10. Adv Immunol. 1994;56:1–26. [PubMed] [Google Scholar]
  22. Panja A., Barone A., Mayer L. Stimulation of lamina propria lymphocytes by intestinal epithelial cells: evidence for recognition of nonclassical restriction elements. J Exp Med. 1994 Mar 1;179(3):943–950. doi: 10.1084/jem.179.3.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Phillips J. O., Everson M. P., Moldoveanu Z., Lue C., Mestecky J. Synergistic effect of IL-4 and IFN-gamma on the expression of polymeric Ig receptor (secretory component) and IgA binding by human epithelial cells. J Immunol. 1990 Sep 15;145(6):1740–1744. [PubMed] [Google Scholar]
  24. Péguet-Navarro J., Moulon C., Caux C., Dalbiez-Gauthier C., Banchereau J., Schmitt D. Interleukin-10 inhibits the primary allogeneic T cell response to human epidermal Langerhans cells. Eur J Immunol. 1994 Apr;24(4):884–891. doi: 10.1002/eji.1830240416. [DOI] [PubMed] [Google Scholar]
  25. Selby W. S., Janossy G., Mason D. Y., Jewell D. P. Expression of HLA-DR antigens by colonic epithelium in inflammatory bowel disease. Clin Exp Immunol. 1983 Sep;53(3):614–618. [PMC free article] [PubMed] [Google Scholar]
  26. Shull M. M., Ormsby I., Kier A. B., Pawlowski S., Diebold R. J., Yin M., Allen R., Sidman C., Proetzel G., Calvin D. Targeted disruption of the mouse transforming growth factor-beta 1 gene results in multifocal inflammatory disease. Nature. 1992 Oct 22;359(6397):693–699. doi: 10.1038/359693a0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Suardet L., Gaide A. C., Calmès J. M., Sordat B., Givel J. C., Eliason J. F., Odartchenko N. Responsiveness of three newly established human colorectal cancer cell lines to transforming growth factors beta 1 and beta 2. Cancer Res. 1992 Jul 1;52(13):3705–3712. [PubMed] [Google Scholar]
  28. Thorsby E. Structure and function of HLA molecules. Transplant Proc. 1987 Feb;19(1 Pt 1):29–35. [PubMed] [Google Scholar]
  29. Zweibaum A., Pinto M., Chevalier G., Dussaulx E., Triadou N., Lacroix B., Haffen K., Brun J. L., Rousset M. Enterocytic differentiation of a subpopulation of the human colon tumor cell line HT-29 selected for growth in sugar-free medium and its inhibition by glucose. J Cell Physiol. 1985 Jan;122(1):21–29. doi: 10.1002/jcp.1041220105. [DOI] [PubMed] [Google Scholar]
  30. de Waal Malefyt R., Abrams J., Bennett B., Figdor C. G., de Vries J. E. Interleukin 10(IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes. J Exp Med. 1991 Nov 1;174(5):1209–1220. doi: 10.1084/jem.174.5.1209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. de Waal Malefyt R., Haanen J., Spits H., Roncarolo M. G., te Velde A., Figdor C., Johnson K., Kastelein R., Yssel H., de Vries J. E. Interleukin 10 (IL-10) and viral IL-10 strongly reduce antigen-specific human T cell proliferation by diminishing the antigen-presenting capacity of monocytes via downregulation of class II major histocompatibility complex expression. J Exp Med. 1991 Oct 1;174(4):915–924. doi: 10.1084/jem.174.4.915. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Mediators of Inflammation are provided here courtesy of Wiley

RESOURCES