Skip to main content
PMC home page
Mediators of Inflammation logoLink to Mediators of Inflammation
. 1998;7(4):283–287. doi: 10.1080/09629359890974

Diminished nitroprusside-induced relaxation of inflamed colonic smooth muscle in mice.

J D van Bergeijk 1, H van Westreenen 1, P Adhien 1, F J Zijlstra 1
PMCID: PMC1781854  PMID: 9792339

Abstract

The dextran sodium sulphate (DSS) induced colitis in mice was used as a experimental model to study the contractility of murine longitudinal colonic smooth muscle during inflammation. Smooth muscle segments of proximal, middle and distal colon were mounted in organ baths. Smooth muscle contraction was induced by carbachol showing an aboral increase in activity, whereas in the inflamed middle colonic segment a marked decrease in activity was observed. The dilatative effect of sodium-nitroprusside (SNP) as a nitric oxide donor was investigated after precontraction by carbachol. Both in normal and DSS segments administration of SNP to isolated mouse colonic smooth muscle preparations caused regional differences in relaxation, the highest relaxation seen in normal proximal colonic tissue. However, this relaxation was markedly reduced in inflamed proximal preparations, associated with a diminished cGMP contents.

Full Text

The Full Text of this article is available as a PDF (153.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnes P. J., Haddad E. B., Rousell J. Regulation of muscarinic M2 receptors. Life Sci. 1997;60(13-14):1015–1021. doi: 10.1016/s0024-3205(97)00042-8. [DOI] [PubMed] [Google Scholar]
  2. Bax W. A., Renzenbrink G. J., van der Linden E. A., Zijlstra F. J., van Heuven-Nolsen D., Fekkes D., Bos E., Saxena P. R. Low-dose aspirin inhibits platelet-induced contraction of the human isolated coronary artery. A role for additional 5-hydroxytryptamine receptor antagonism against coronary vasospasm? Circulation. 1994 Feb;89(2):623–629. doi: 10.1161/01.cir.89.2.623. [DOI] [PubMed] [Google Scholar]
  3. Boughton-Smith N. K., Evans S. M., Hawkey C. J., Cole A. T., Balsitis M., Whittle B. J., Moncada S. Nitric oxide synthase activity in ulcerative colitis and Crohn's disease. Lancet. 1993 Aug 7;342(8867):338–340. doi: 10.1016/0140-6736(93)91476-3. [DOI] [PubMed] [Google Scholar]
  4. Calignano A., Whittle B. J., Di Rosa M., Moncada S. Involvement of endogenous nitric oxide in the regulation of rat intestinal motility in vivo. Eur J Pharmacol. 1992 Dec 15;229(2-3):273–276. doi: 10.1016/0014-2999(92)90567-n. [DOI] [PubMed] [Google Scholar]
  5. Dieleman L. A., Ridwan B. U., Tennyson G. S., Beagley K. W., Bucy R. P., Elson C. O. Dextran sulfate sodium-induced colitis occurs in severe combined immunodeficient mice. Gastroenterology. 1994 Dec;107(6):1643–1652. doi: 10.1016/0016-5085(94)90803-6. [DOI] [PubMed] [Google Scholar]
  6. Iversen H. H., Wiklund N. P., Olgart C., Gustafsson L. E. Nerve stimulation-induced nitric oxide release as a consequence of muscarinic M1 receptor activation. Eur J Pharmacol. 1997 Jul 23;331(2-3):213–219. doi: 10.1016/s0014-2999(97)01027-3. [DOI] [PubMed] [Google Scholar]
  7. Maehara T., Fujita A., Suthamnatpong N., Takeuchi T., Hata F. Differences in relaxant effects of cyclic GMP on skinned muscle preparations from the proximal and distal colon of rats. Eur J Pharmacol. 1994 Aug 11;261(1-2):163–170. doi: 10.1016/0014-2999(94)90315-8. [DOI] [PubMed] [Google Scholar]
  8. Martinolle J. P., Garcia-Villar R., Fioramonti J., Bueno L. Altered contractility of circular and longitudinal muscle in TNBS-inflamed guinea pig ileum. Am J Physiol. 1997 May;272(5 Pt 1):G1258–G1267. doi: 10.1152/ajpgi.1997.272.5.G1258. [DOI] [PubMed] [Google Scholar]
  9. Middleton S. J., Shorthouse M., Hunter J. O. Increased nitric oxide synthesis in ulcerative colitis. Lancet. 1993 Feb 20;341(8843):465–466. doi: 10.1016/0140-6736(93)90211-x. [DOI] [PubMed] [Google Scholar]
  10. Ohkusa T., Okayasu I., Tokoi S., Araki A., Ozaki Y. Changes in bacterial phagocytosis of macrophages in experimental ulcerative colitis. Digestion. 1995;56(2):159–164. doi: 10.1159/000201236. [DOI] [PubMed] [Google Scholar]
  11. Okayasu I., Hatakeyama S., Yamada M., Ohkusa T., Inagaki Y., Nakaya R. A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterology. 1990 Mar;98(3):694–702. doi: 10.1016/0016-5085(90)90290-h. [DOI] [PubMed] [Google Scholar]
  12. Rachmilewitz D., Stamler J. S., Bachwich D., Karmeli F., Ackerman Z., Podolsky D. K. Enhanced colonic nitric oxide generation and nitric oxide synthase activity in ulcerative colitis and Crohn's disease. Gut. 1995 May;36(5):718–723. doi: 10.1136/gut.36.5.718. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Suthamnatpong N., Hata F., Kanada A., Takeuchi T., Yagasaki O. Mediators of nonadrenergic, noncholinergic inhibition in the proximal, middle and distal regions of rat colon. Br J Pharmacol. 1993 Feb;108(2):348–355. doi: 10.1111/j.1476-5381.1993.tb12808.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tepperman B. L., Brown J. F., Whittle B. J. Nitric oxide synthase induction and intestinal epithelial cell viability in rats. Am J Physiol. 1993 Aug;265(2 Pt 1):G214–G218. doi: 10.1152/ajpgi.1993.265.2.G214. [DOI] [PubMed] [Google Scholar]

Articles from Mediators of Inflammation are provided here courtesy of Wiley

RESOURCES