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. 1996 Jul;178(14):4313–4318. doi: 10.1128/jb.178.14.4313-4318.1996

Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide.

F Katzen 1, A Becker 1, A Zorreguieta 1, A Pühler 1, L Ielpi 1
PMCID: PMC178194  PMID: 8763965

Abstract

The Xanthomonas campestris gum gene cluster is composed of 12 genes designated gumB, -C, -D, -E, -F, -G, -H, -I, -J, -K, -L, and -M. The transcriptional organization of this gene cluster was analyzed by the construction of gum-lacZ transcriptional fusions in association with plasmid integration mutagenesis. This analysis, coupled with primer extension assays, indicated that the gum region was mainly expressed as an operon from a promoter located upstream of the first gene, gumB.

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Selected References

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