Table 1.
Name/gene | Primer orientation | Primer sequence | GenBank accession no. |
---|---|---|---|
TLR1 | Forward | 5′-ACATCAAGTGTGTGCTTGAA-3′ | AF316985 |
Reverse | 5′-CCCCATCACTGTACCTTAGA-3′ | ||
TLR2 | Forward | 5′-ACAGCTACTGTGTGACTCTCCGCC-3′ | AF124741 |
Reverse | 5′-GGTCTTGGTGTTCATTATCTTGCGC-3′ | ||
TLR4 | Forward | 5′-GACCTCAGCTTCAATGGTGC-3′ | AF095353 |
Reverse | 5′-TATCAGAAATGCTACAGTGGATACC-3′ | ||
TLR6 | Forward | 5′-ACATCTCTTGCTGCCCTATG-3′ | AF314636 |
Reverse | 5′-GAGGAACACTTGGTTTTTGAC-3′ | ||
MyD88 | Forward | 5′-CACCTGTGTCTGGTCCATT-3′ | MMU84409 |
Reverse | 5′-CGCAGGATACTGGGAAAGT-3′ | ||
NF-κB | Forward | 5′-TGGCTACTATGAGGCTGACC-3′ | AF199371 |
Reverse | 5′-GTTGATGGTGCTGAGGGAT-3′ | ||
GAPDH | Forward | 5′-CCATGGAGAAGGCTGGGG-3′ | NM_001001303 |
Reverse | 5′-CAAAGTTGTCATGGATGACC-3′ |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MyD88, myeloid differentiation factor 88; NF-κB, nuclear factor-kappa B; TLR, Toll-like receptors.