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The level of autoantibodies in MRL/lpr mice over time with age. Sera obtained from five BALB/c and five MRL/lpr mice at different time-points were tested for anti-dsDNA IgG (a) and anti- U1A IgG (b) by ELISA. Sera were diluted 1 ≕ 100 for detecting these two kinds of autoantibodies. Values that were greater than the mean + 3SD (horizontal dash line) from 4-month-old BALB/c mice (n = 5) were regarded as positive. *Indicates P < 0·01 when compared to age-matched BALB/c mice.
