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Constraints of treatment with EtxB 10–12-week old female NOD mice (12 per group) were treated either intranasally or subcutaneously with 10 µg EtxB(a; ○), with 10 10 µg EtxB(G33D)(b;•) or were given PBS alone (▪) on the number of occasions indicated. Diabetes was diagnosed on the basis of weekly urinary glucose testing
