Figure 3.

The dual altered peptide ligand (APL) up-regulates phosphorylation of pJNK and p-38 in p195–212-specific T cells. T cells (5 × 10⁶) of the line were stimulated with p195–212 with or without the dual or the reversed dual APL for 24 hr. Whole-cell lysates were prepared, separated on sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE), and transferred to a nitrocellulose membrane that was later blotted with anti-p-JNK or anti-p-38 or their respective totals. Results are expressed as densitometry units (measured with National Institutes of Health image software). Shown is one of two experiments performed.