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Ex vivo cytolytic assay. The same clonotypic CD8 T cells harvested from the experiments outlined in Fig. 6 were assayed for their cytolytic activity on A20 lymphoma targets (H-2d) pulsed with Kd-HA peptide (A20-HA) or an irrelevant peptide (A20-Con) by a 6-hr JAM test. Percentage of specific lysis as a function of effector to target ratio is indicated. Representative data of three independent experiments are shown.
