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. 2006 Jul;118(3):353–360. doi: 10.1111/j.1365-2567.2006.02379.x

Figure 1.

Figure 1

High up-regulation of ICOS-L on mature PDC. (a) Human PDC were isolated from the peripheral blood and analysed by flow cytometry for the expression of CD80, CD86, ICOS-L, MHC class-II and the IL-3 receptor α-chain (CD123) immediately after isolation or after maturation in the combined presence of IL-3 and a CD40L-expressing transfectant for 48 hr. One representative experiment of five is shown. (b) Human immature monocyte-derived DC (moDC) and mature moDC were analysed for the expression of the same markers for comparison. Black histograms represent isotype control staining, inset numbers indicate the mean fluorescence over all cells minus the isotype control mean fluorescence.