Relative cytokine production in cocultures of T cells with mature PDC or mature moDC. Mature PDC or mature moDC were cocultured with CD4+ CD45RO+ T cells from a given donor for 48 hr in the presence of SEB, and cytokine levels in culture supernatants were determined by ELISA. In each comparative experiment the level of each cytokine in the PDC/T-cell coculture was set as 100%. The data shown represent the average (± SEM) of three to five comparative experiments. In a typical comparative experiment the following absolute cytokine levels were measured in the T/PDC coculture: IL-3, 73 ng/ml; IL-4, 704 pg/ml; IL-10, 8 ng/ml; IL-5, 210 pg/ml; IL-2, 47 ng/ml; IL-6, 803 pg/ml; IL-13, 1371 pg/ml; IFN-γ, 1806 pg/ml; and in the T/moDC coculture: IL-3, 19 ng/ml; IL-4, 194 pg/ml; IL-10, 1·5 ng/ml; IL-5, 581 pg/ml; IL-2, 29 ng/ml; IL-6, 558 pg/ml; IL-13, 1513 pg/ml; IFN-γ, 1432 pg/ml.