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. 2006 Sep 1;119(1):8–17. doi: 10.1111/j.1365-2567.2006.02383.x

Figure 6.

Figure 6

SOCS3-regulation of IL-10 activity occurs via a STAT3-independent mechanism. (a) Uninfected monocytes were exposed to 10 ng/ml IL-10, 500 ng/ml LPS or LPS with IL-10 for 0–120 min. (b) Monocytes were infected with AdV-GFP or AdV-SOCS3 at MOI 50 for 24 hr before being stimulated with LPS, or (c) LPS + IL-10 for 0–120 min. Western blot analysis was performed (7·5 µg lysate/lane) and the kinetics of STAT3 phosphorylation determined. Over-expression of SOCS3 by AdV-SOCS3-infected cells was confirmed using an anti-SOCS3 antibody. Protein loading was visualized using anti-β-tubulin. The blots shown are representative of three independent experiments using monocytes harvested from different donors.