Erratum

Anti-idiotype antibody induced cellular immunity in mice transgenic for human carcinoembryonic antigen

Asim Saha, Sunil K. Chatterjee, Kenneth A. Foon and Malaya Bhattacharya-Chatterjee

Table 1 in the above mentioned paper¹ contained some errors. The correct table is printed below. The publishers apologise for this error.

Table 1.

Cytotoxicity and cytokine production by CTL on coincubation with various cell lines¹

Tumour cells	Histology	MHC class I	mAb	Lysis (%)	IFN-γ (pg/ml)	TNF-α (pg/ml)	IL-4 (pg/ml)
C15	Colon carcinoma	+	Isotype control	48.7 ± 4.1	2000 ± 40*	450 ± 30*	<20
			Anti-CD4	40.0 ± 3.9	1260 ± 30	285 ± 15	<20
			Anti-CD8	9.8 ± 1.8	360 ± 10	180 ± 15	<20
			Anti-I-Ab	38.9 ± 4.2	1190 ± 25	270 ± 15	<20
			Anti-H-2Kb/H-2Db	10.9 ± 2.1	425 ± 15	165 ± 15	<20
MC-38	Colon carcinoma	+		10.8 ± 2.4	1105 ± 25	180 ± 15	<20
EL4	Thymoma	+		4.3 ± 2.0	1075 ± 20	135 ± 15	<10
RMA	Lymphoma	+		10.9 ± 2.6	1030 ± 25	150 ± 15	<10
B16-F10	Melanoma	–		7.5 ± 2.1	990 ± 20	135 ± 15	<10
SCC VII	Squamous cell carcinoma	+		10.6 ± 3.0	885 ± 20	135 ± 15	<10
AG104A	Fibrosarcoma	+		2.3 ± 1.6	955 ± 20	150 ± 15	<10
TSA	Mammary carcinoma	+		5.2 ± 2.0	825 ± 15	165 ± 15	<10
YAC-1	Lymphoma	ND		8.8 ± 2.8	1010 ± 20	150 ± 15	<10
–					1090 ± 25	165 ± 15	<10

¹Cytotoxicity against various cell lines was measured in a 6 hr ⁵¹Cr release assay at an E : T ratio of 25 : 1. CTL were generated from 3H1-pulsed DC immunized mice as described. CTL (2 × 10⁵/well) were incubated with tumour cells (2 × 10⁴/well) or media alone in 96-well tissue culture plates at 37° for 6 hr. Cell-free supernatants were harvested and analysed for the production of IFN-γ, TNF-α, and IL-4 by standard ELISA kits.¹⁷ For inhibition experiments, blocking mAb was added to the culture at a concentration of 10 µg/ml. Surface MHC class I antigen expression was analysed by flow cytometry. The representative data of three independent experiments is shown.

^*P < 0.002 with respect to other culture conditions for IFN-γ and TNF-α production. ND, not determined.