Figure 1.
Effect of NF-κB inhibitors on cytokine-dependent increases in pIgR mRNA. HT29 cells were preincubated for 1 hr without and with 5 μm Na2SeO3 (Se), 30 μg/ml CAPE, 50 μm Bay 11-7085 (Bay), or 5 μm MG115 and then with 10 ng/ml IL-4, 200 U/ml IFN-γ or both together for 24 hr. Total RNA was isolated and pIgR mRNA was determined by RPA. Densitometric analysis of autoradiographs was performed as described in Materials and Methods and the percent decrease relative to cultures without inhibitor treatment was determined. Values represent the mean ± SEM for combined data from three to six independent experiments for each condition. A significant decrease in steady state mRNA levels was observed in inhibitor-treated cells relative to controls: *P < 0.001, **P < 0.01.