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CD4+ T-cell lines proliferate in response to T2-B27 (a T2-cell line transfected with HLA-B*2705). (a) Proliferation is expressed as Δ counts per minute (c.p.m.) of CD4+ T-cell lines to a panel of B27+ and B27− stimulator cell lines. Data shown are the means of duplicate wells ± standard deviation (SD). (b) Flow cytometric analysis of the phenotype of the six T2-B27-reactive T-cell lines.
