Table 2.
IFN-α (U/ml) | TNF-α (pg/ml) | |
---|---|---|
Medium | <3 | <5 |
CpG-ODN D32 | 19 032 ± 840 | 841 ± 82 |
CpG-ODN 2216 | 12 568 ± 890 | 585 ± 84 |
CpG ODN 2006 | 123 ± 9 | <5 |
LPS | 106 ± 4 | 415 ± 44 |
pIC | 99 ± 2 | <5 |
The SWC3+-sorted cells were stimulated with CpG-oligonucleotides (ODNs) (10 µg/ml), lipopolysaccharide (LPS) (1 µg/ml) or Poly I : C (pIC) (10 µg/ml). The cell supernatants were tested for the presence of interferon-α(IFN-α)and tumour necrosis factor-α(TNF-α) by enzyme-linked immunosorbent assay (ELISA) at 24 hr poststimulation.