Figure 5.

ISS-ODN effects on cysteinyl-leukotriene release in vivo and in vitro. (a) Mice (four mice/group) were sensitized and challenged as outlined in Fig. 1. Fifteen min after the final i.n. OVA challenge, NLF samples were collected to measure total cysteinyl leukotriene levels. (b) BMDMs were incubated with ISS-ODN, C-ODN, or were left unstimulated for 24 hr. RNA was then harvested for Northern blot analysis of LTC₄ synthase mRNA expression. Equivalence in RNA loading was established by comparing methylene blue staining of 28S RNA on the membranes. (c) BMDMs described in (b) were activated with calcium ionophore for an additional 15 min before supernatants were harvested for cysteinyl leukotriene determinations. All results are reflective of three separate experiments (*P ≤ 0·05 for comparisons to OVA sensitized and challenged mice receiving no intervention).