Figure 7.

Combination of interleukin (IL)-4 and IL-10 suppressed the production of IL-12 in vitro. The single cell suspensions of splenocytes from naive BALB/c mice were stimulated with live Leishmania major in complete RPMI-1640 media for 24 hr. The cells were also stimulated with lipopolysaccharide (LPS) as the control. For the inhibitory efficacy of IL-4 and IL-10 on the production of IL-12, the cells were cultured in the presence of recombinant IL-4 and rIL-10. After culture, the supernatants were collected and the IL-12 (p40) levels were quantified using an enzyme-linked immunosorbent assay (ELISA). The data shown represent the mean ± standard deviation (SD) of triplicate experiments. *P < 0·001 in comparison to the controls which were stimulated with L. major in the absence of IL-4 and IL-10.