Figure 2.

Cross-linking of G_M1 does not influence the presentation of fluid-phase or B-cell receptor (BCR)-internalized antigen. A20µWT cells [transfected with anti-phosphorylcholine (anti-PC) antibody] were incubated with increasing concentrations of either free ovalbumin (OVA) (panels a and c) or PC-OVA (panels b and d) in the presence or absence of 30 µg/ml of Escherichia coli enterotoxin B subunit (EtxB), for 24 hr (panels a and b) or 2 hr (panels c and d) at 37°. DO.11 cells were subsequently added in the continual presence of antigens, and incubated for 18–20 hr at 37°. Supernatants were analysed with interleukin-2 (IL-2)-responsive HT-2 cells. Data represent mean proliferation of quadruplicate samples ± standard error of the mean (SEM). The data represent three similar independent experiments. c.p.m., counts per minute.