Figure 6.

Ligation of the B-cell receptor (BCR) does not accelerate antigen presentation via G_M1. A20µWT cells were preincubated with 40 µg/ml of Escherichia coli enterotoxin B subunit-ovalbumin (EtxB-OVA) for 10 min to allow binding. To ligate the BCR, phosphorylcholine-bovine serum albumin (PC-BSA) conjugate (at 100 µg/ml) was subsequently added. Cells were then incubated at 37° with the antigens for 0–240 min, washed and fixed with 1% paraformaldehyde. DO.11 cells were added to antigen-pulsed and fixed antigen-presenting cells (APC) and incubated for 18–20 hr. Supernatants were analysed with interleukin-2 (IL-2)-responsive HT-2 cells. Data represent mean proliferation of quadruplicate samples±standard error of the mean (SEM). c.p.m., counts per minute.