Table 1. Specificity of monoclonal antibodies (mAbs) against guinea-pig CD1 proteins.
| Guinea-pig CD1 isoforms | ||||||||
|---|---|---|---|---|---|---|---|---|
| mAbs | Derived against | b1 | b2 | b3 | b4 | c1 | c2 | c3 |
| CD1F2/5E3 | Guinea-pig CD1 | − | ± | ++ | + | ++ | ++ | + |
| CD1F2/6B5 | Guinea-pig CD1 | + | ++ | ++ | + | ++ | ++ | + |
| CD1F2/1B12 | Guinea-pig CD1 | − | ++ | ++ | ++ | − | − | + |
| CD1·4-1D12 | Guinea-pig CD1 | + | − | − | − | − | − | − |
| Msgp9 | Guinea-pig CD1 | − | − | ++ | − | − | − | − |
| 7C4 | Human CD1b | − | + | + | − | − | − | − |
| WM25 | Human CD1b | − | − | ++ | − | − | − | − |
| BCD1b1 | Human CD1b | − | − | ++ | + | − | − | − |
| BCD1b2 | Human CD1b | − | ++ | ++ | ++ | − | − | + |
| BCD1b3 | Human CD1b | − | ++ | ++ | ++ | ± | − | + |
| BCD1b4 | Human CD1b | − | ++ | ++ | − | − | − | − |
| CD1d69 | Human CD1d | − | − | − | ±∼ +* | − | − | − |
| 20–27 | Sheep CD1 | − | − | ++ | ++ | ± | − | − |
| CC-20 | Cow CD1 | − | ++ | + | ++ | − | − | − |
| Fe5·5C1 | Feline CD1 | − | − | ++ | ± | ++ | ++ | + |
New mAbs generated in this study and mAbs against CD1 of human or other species were tested for their reactivity with guinea-pig CD1 (gpCD1) proteins using transfectants of guinea-pig cell lines 104C1 and GPC-16 expressing individual isoforms of gpCD1. All mAbs were mouse immunoglobulin G (IgG) and were detected with anti-mouse IgG/IgM-conjugated to fluorescein isothiocyanate (FITC) as a secondary reagent. Symbols signify the following: ‘−’, no reactivity above the level of isotype-matched non-binding control antibody; ‘±’, weak but significant shift from isotype-matched control antibody; ‘+’, positive staining with a mean fluorescence intensity (MFI) of 10–100; ‘++’, strong staining with a MFI of >100.
gpCD1b4 expression detected by CD1d69 was ‘+’ in GPC-16 cells transfected with gpCD1b4 and ‘±’ in 104C1 transfectant cells.