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. 2002 Jun;106(2):200–211. doi: 10.1046/j.1365-2567.2002.01414.x

Figure 3.

Figure 3

Effect of ΔlytB low molecular weight extract on a range of immunological parameters. Peripheral blood mononuclear cells (PBMC) were incubated with medium alone, with isopentenyl pyrophosphate (IPP) at 2 µm, or with LMW extract prepared from wild-type (WT) Escherichia coli, ΔgcpE, or ΔlytB, at a dilution of 1 : 62 500. Data represent results from one donor, for expression of CD25 (day 3), CD69 (day 1), CD94 (day 6) and human leucocyte antigen (HLA)-DR (day 6) on Vγ9+ T cells, and secretion of tumour necrosis factor (TNF-α) (day 1) and interferon-γ (IFN-γ) (day 3) by PBMC. Note: the pattern shown is typical for a number of blood donors analysed (n = 3–6); however, with absolute levels of CD94 and HLA-DR expression, as well as absolute amounts of cytokines secreted, greater biological variation was found between individuals than in the data for γδ T-cell expansion and expression of CD25 or CD69. Also, for some donors changes in CD94 and HLA-DR expression in the presence of serial dilutions of phosphoantigens was inconsistent despite good dose-dependent responses, as judged by CD25 and CD69 expression, thus making CD25 and CD69 the more reproducible activation markers tested.