Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1996 Aug;178(16):5027–5031. doi: 10.1128/jb.178.16.5027-5031.1996

Isolation and characterization of the structural gene for OmpL, a pressure-regulated porin-like protein from the deep-sea bacterium Photobacterium species strain SS9.

T J Welch 1, D H Bartlett 1
PMCID: PMC178291  PMID: 8759872

Abstract

Transposon-directed cloning was used to isolate the ompL gene from the deep-sea bacterium Photobacterium species strain SS9. The deduced amino acid sequence of OmpL displays sequence homology to porin proteins from enteric bacteria. Gene fusion and primer extension analyses indicate that ompL is transcriptionally regulated by pressure.

Full Text

The Full Text of this article is available as a PDF (313.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. doi: 10.1016/S0022-2836(05)80360-2. [DOI] [PubMed] [Google Scholar]
  2. Bartlett D. H., Chi E., Wright M. E. Sequence of the ompH gene from the deep-sea bacterium Photobacterium SS9. Gene. 1993 Sep 6;131(1):125–128. doi: 10.1016/0378-1119(93)90680-2. [DOI] [PubMed] [Google Scholar]
  3. Bartlett D. H. Microbial life at high pressures. Sci Prog. 1992;76(301-302):479–496. [PubMed] [Google Scholar]
  4. Bartlett D. H., Welch T. J. ompH gene expression is regulated by multiple environmental cues in addition to high pressure in the deep-sea bacterium Photobacterium species strain SS9. J Bacteriol. 1995 Feb;177(4):1008–1016. doi: 10.1128/jb.177.4.1008-1016.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bartlett D., Chi E. Genetic characterization of ompH mutants in the deep-sea bacterium Photobacterium sp. strain SS9. Arch Microbiol. 1994;162(5):323–328. doi: 10.1007/BF00263779. [DOI] [PubMed] [Google Scholar]
  6. Bartlett D., Wright M., Yayanos A. A., Silverman M. Isolation of a gene regulated by hydrostatic pressure in a deep-sea bacterium. Nature. 1989 Nov 30;342(6249):572–574. doi: 10.1038/342572a0. [DOI] [PubMed] [Google Scholar]
  7. Bauer K., Struyvé M., Bosch D., Benz R., Tommassen J. One single lysine residue is responsible for the special interaction between polyphosphate and the outer membrane porin PhoE of Escherichia coli. J Biol Chem. 1989 Oct 5;264(28):16393–16398. [PubMed] [Google Scholar]
  8. Chi E., Bartlett D. H. Use of a reporter gene to follow high-pressure signal transduction in the deep-sea bacterium Photobacterium sp. strain SS9. J Bacteriol. 1993 Dec;175(23):7533–7540. doi: 10.1128/jb.175.23.7533-7540.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Chou P. Y., Fasman G. D. Prediction of the secondary structure of proteins from their amino acid sequence. Adv Enzymol Relat Areas Mol Biol. 1978;47:45–148. doi: 10.1002/9780470122921.ch2. [DOI] [PubMed] [Google Scholar]
  10. Elkins C., Carbonetti N. H., Coímbre A. J., Thomas C. E., Sparling P. F. Cloning and constitutive expression of structural genes encoding gonococcal porin protein in Escherichia coli and attenuated Salmonella typhimurium vaccine strains. Gene. 1994 Jan 28;138(1-2):43–50. doi: 10.1016/0378-1119(94)90781-1. [DOI] [PubMed] [Google Scholar]
  11. Graf J., Dunlap P. V., Ruby E. G. Effect of transposon-induced motility mutations on colonization of the host light organ by Vibrio fischeri. J Bacteriol. 1994 Nov;176(22):6986–6991. doi: 10.1128/jb.176.22.6986-6991.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Granger B. L., Lazarides E. Desmin and vimentin coexist at the periphery of the myofibril Z disc. Cell. 1979 Dec;18(4):1053–1063. doi: 10.1016/0092-8674(79)90218-6. [DOI] [PubMed] [Google Scholar]
  13. Jeanteur D., Lakey J. H., Pattus F. The bacterial porin superfamily: sequence alignment and structure prediction. Mol Microbiol. 1991 Sep;5(9):2153–2164. doi: 10.1111/j.1365-2958.1991.tb02145.x. [DOI] [PubMed] [Google Scholar]
  14. Lipman D. J., Pearson W. R. Rapid and sensitive protein similarity searches. Science. 1985 Mar 22;227(4693):1435–1441. doi: 10.1126/science.2983426. [DOI] [PubMed] [Google Scholar]
  15. Mizuno T., Chou M. Y., Inouye M. A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA). Proc Natl Acad Sci U S A. 1984 Apr;81(7):1966–1970. doi: 10.1073/pnas.81.7.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nikaido H., Vaara M. Molecular basis of bacterial outer membrane permeability. Microbiol Rev. 1985 Mar;49(1):1–32. doi: 10.1128/mr.49.1.1-32.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Norrander J., Kempe T., Messing J. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene. 1983 Dec;26(1):101–106. doi: 10.1016/0378-1119(83)90040-9. [DOI] [PubMed] [Google Scholar]
  18. Oliver D. Protein secretion in Escherichia coli. Annu Rev Microbiol. 1985;39:615–648. doi: 10.1146/annurev.mi.39.100185.003151. [DOI] [PubMed] [Google Scholar]
  19. Struyvé M., Moons M., Tommassen J. Carboxy-terminal phenylalanine is essential for the correct assembly of a bacterial outer membrane protein. J Mol Biol. 1991 Mar 5;218(1):141–148. doi: 10.1016/0022-2836(91)90880-f. [DOI] [PubMed] [Google Scholar]
  20. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Welch T. J., Farewell A., Neidhardt F. C., Bartlett D. H. Stress response of Escherichia coli to elevated hydrostatic pressure. J Bacteriol. 1993 Nov;175(22):7170–7177. doi: 10.1128/jb.175.22.7170-7177.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES