Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1996 Aug;178(16):5042–5044. doi: 10.1128/jb.178.16.5042-5044.1996

Single-stranded DNA-binding protein enhances the stability of CTG triplet repeats in Escherichia coli.

W A Rosche 1, A Jaworski 1, S Kang 1, S F Kramer 1, J E Larson 1, D P Geidroc 1, R D Wells 1, R R Sinden 1
PMCID: PMC178294  PMID: 8759875

Abstract

The stability of CTG triplet repeats was analyzed in Escherichia coli to identify processes responsible for their genetic instability. Using a biochemical assay for stability, we show that the absence of single-stranded-DNA-binding protein leads to an increase in the frequency of large deletions within the triplet repeats.

Full Text

The Full Text of this article is available as a PDF (354.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bebenek K., Abbotts J., Roberts J. D., Wilson S. H., Kunkel T. A. Specificity and mechanism of error-prone replication by human immunodeficiency virus-1 reverse transcriptase. J Biol Chem. 1989 Oct 5;264(28):16948–16956. [PubMed] [Google Scholar]
  2. Bierne H., Ehrlich S. D., Michel B. The replication termination signal terB of the Escherichia coli chromosome is a deletion hot spot. EMBO J. 1991 Sep;10(9):2699–2705. doi: 10.1002/j.1460-2075.1991.tb07814.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Edwards A., Hammond H. A., Jin L., Caskey C. T., Chakraborty R. Genetic variation at five trimeric and tetrameric tandem repeat loci in four human population groups. Genomics. 1992 Feb;12(2):241–253. doi: 10.1016/0888-7543(92)90371-x. [DOI] [PubMed] [Google Scholar]
  4. Fishel R., Lescoe M. K., Rao M. R., Copeland N. G., Jenkins N. A., Garber J., Kane M., Kolodner R. The human mutator gene homolog MSH2 and its association with hereditary nonpolyposis colon cancer. Cell. 1993 Dec 3;75(5):1027–1038. doi: 10.1016/0092-8674(93)90546-3. [DOI] [PubMed] [Google Scholar]
  5. Gacy A. M., Goellner G., Juranić N., Macura S., McMurray C. T. Trinucleotide repeats that expand in human disease form hairpin structures in vitro. Cell. 1995 May 19;81(4):533–540. doi: 10.1016/0092-8674(95)90074-8. [DOI] [PubMed] [Google Scholar]
  6. Glassberg J., Meyer R. R., Kornberg A. Mutant single-strand binding protein of Escherichia coli: genetic and physiological characterization. J Bacteriol. 1979 Oct;140(1):14–19. doi: 10.1128/jb.140.1.14-19.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hacker K. J., Alberts B. M. The rapid dissociation of the T4 DNA polymerase holoenzyme when stopped by a DNA hairpin helix. A model for polymerase release following the termination of each Okazaki fragment. J Biol Chem. 1994 Sep 30;269(39):24221–24228. [PubMed] [Google Scholar]
  8. Jaworski A., Rosche W. A., Gellibolian R., Kang S., Shimizu M., Bowater R. P., Sinden R. R., Wells R. D. Mismatch repair in Escherichia coli enhances instability of (CTG)n triplet repeats from human hereditary diseases. Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):11019–11023. doi: 10.1073/pnas.92.24.11019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kang S., Jaworski A., Ohshima K., Wells R. D. Expansion and deletion of CTG repeats from human disease genes are determined by the direction of replication in E. coli. Nat Genet. 1995 Jun;10(2):213–218. doi: 10.1038/ng0695-213. [DOI] [PubMed] [Google Scholar]
  10. Leach D. R. Long DNA palindromes, cruciform structures, genetic instability and secondary structure repair. Bioessays. 1994 Dec;16(12):893–900. doi: 10.1002/bies.950161207. [DOI] [PubMed] [Google Scholar]
  11. Leach F. S., Nicolaides N. C., Papadopoulos N., Liu B., Jen J., Parsons R., Peltomäki P., Sistonen P., Aaltonen L. A., Nyström-Lahti M. Mutations of a mutS homolog in hereditary nonpolyposis colorectal cancer. Cell. 1993 Dec 17;75(6):1215–1225. doi: 10.1016/0092-8674(93)90330-s. [DOI] [PubMed] [Google Scholar]
  12. Levinson G., Gutman G. A. Slipped-strand mispairing: a major mechanism for DNA sequence evolution. Mol Biol Evol. 1987 May;4(3):203–221. doi: 10.1093/oxfordjournals.molbev.a040442. [DOI] [PubMed] [Google Scholar]
  13. Lohman T. M., Ferrari M. E. Escherichia coli single-stranded DNA-binding protein: multiple DNA-binding modes and cooperativities. Annu Rev Biochem. 1994;63:527–570. doi: 10.1146/annurev.bi.63.070194.002523. [DOI] [PubMed] [Google Scholar]
  14. Meyer R. R., Glassberg J., Kornberg A. An Escherichia coli mutant defective in single-strand binding protein is defective in DNA replication. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1702–1705. doi: 10.1073/pnas.76.4.1702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Meyer R. R., Glassberg J., Scott J. V., Kornberg A. A temperature-sensitive single-stranded DNA-binding protein from Escherichia coli. J Biol Chem. 1980 Apr 10;255(7):2897–2901. [PubMed] [Google Scholar]
  16. Papanicolaou C., Ripley L. S. An in vitro approach to identifying specificity determinants of mutagenesis mediated by DNA misalignments. J Mol Biol. 1991 Oct 5;221(3):805–821. doi: 10.1016/0022-2836(91)80177-v. [DOI] [PubMed] [Google Scholar]
  17. Samadashwily G. M., Mirkin S. M. Trapping DNA polymerases using triplex-forming oligodeoxyribonucleotides. Gene. 1994 Nov 4;149(1):127–136. doi: 10.1016/0378-1119(94)90421-9. [DOI] [PubMed] [Google Scholar]
  18. Sinden R. R., Wells R. D. DNA structure, mutations, and human genetic disease. Curr Opin Biotechnol. 1992 Dec;3(6):612–622. doi: 10.1016/0958-1669(92)90005-4. [DOI] [PubMed] [Google Scholar]
  19. Sinden R. R., Zheng G. X., Brankamp R. G., Allen K. N. On the deletion of inverted repeated DNA in Escherichia coli: effects of length, thermal stability, and cruciform formation in vivo. Genetics. 1991 Dec;129(4):991–1005. doi: 10.1093/genetics/129.4.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Smith G. K., Jie J., Fox G. E., Gao X. DNA CTG triplet repeats involved in dynamic mutations of neurologically related gene sequences form stable duplexes. Nucleic Acids Res. 1995 Nov 11;23(21):4303–4311. doi: 10.1093/nar/23.21.4303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Strand M., Prolla T. A., Liskay R. M., Petes T. D. Destabilization of tracts of simple repetitive DNA in yeast by mutations affecting DNA mismatch repair. Nature. 1993 Sep 16;365(6443):274–276. doi: 10.1038/365274a0. [DOI] [PubMed] [Google Scholar]
  22. Sutherland G. R., Richards R. I. Simple tandem DNA repeats and human genetic disease. Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3636–3641. doi: 10.1073/pnas.92.9.3636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Weaver D. T., DePamphilis M. L. The role of palindromic and non-palindromic sequences in arresting DNA synthesis in vitro and in vivo. J Mol Biol. 1984 Dec 25;180(4):961–986. doi: 10.1016/0022-2836(84)90266-3. [DOI] [PubMed] [Google Scholar]
  24. Wells R. D. Molecular basis of genetic instability of triplet repeats. J Biol Chem. 1996 Feb 9;271(6):2875–2878. doi: 10.1074/jbc.271.6.2875. [DOI] [PubMed] [Google Scholar]
  25. Yu A., Dill J., Wirth S. S., Huang G., Lee V. H., Haworth I. S., Mitas M. The trinucleotide repeat sequence d(GTC)15 adopts a hairpin conformation. Nucleic Acids Res. 1995 Jul 25;23(14):2706–2714. doi: 10.1093/nar/23.14.2706. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES