Figure 1.
Effects of LY294002 on B-cell proliferation driven by various stimuli. BALB/c B cells (2 × 105) were preincubated with different concentrations of LY294002 for 30 min before the addition of lipopolysaccharide (LPS) (10 µg/ml), F(ab′)2 anti-µ (10 µg/ml), anti-CD40 (10 µg/ml), anti-CD38 (1 : 200 dilution of s/n) or phorbol 12-myristate 13-acetate (PMA) (5 ng/ml) + ionomycin (1 µm). After 68 hr, the cultures were pulsed with [3H]thymidine and harvested 4 h later. Data shown are derived from the means of triplicate cultures and represent the inhibition produced by each concentration of inhibitor compared with control cultures lacking LY294002. Uptakes ranged from 7500 counts per minute (c.p.m.) (control stimulated with anti-CD38) to 85 775 c.p.m. (LPS controls). The data shown are representative of three similar experiments.