Figure 1.

Effects of anti-OX40 ligand (OX40L), anti-human leucocyte antigen (HLA)-DR, anti-CD18 and cytotoxic T lymphocyte-associated antigen-4 (CTLA-4)-Fc fusion protein on the two-way mixed leucocyte reaction (MLR). Peripheral blood mononuclear cells (PBMC) from two different donors were mixed and co-cultured in a 96-well culture plate for 7 days in the presence of the indicated reagents. Cells were pulsed with [³H]thymidine ([³H]TdR) (0·5 µCi/well) for the last 7 hr of culture and the incorporated radioactivity was measured in a liquid scintillation counter. Results are expressed as mean counts ± standard deviation (SD) of triplicate samples. The asterisk indicates a P-value of < 0·05 compared with the suppressed reactivity.