Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1996 Aug;178(16):5049–5052. doi: 10.1128/jb.178.16.5049-5052.1996

A developmental stage-specific histone H1 homolog of Coxiella burnetii.

R A Heinzen 1, T Hackstadt 1
PMCID: PMC178296  PMID: 8759877

Abstract

Two DNA-binding proteins have been detected in Coxiella burnetii by southwestern (DNA-protein) blotting. One of these, termed Hq1, is enriched in the small cell variant stage of the developmental cycle and displays compositional and primary amino acid sequence similarities to eukaryotic histone H1. C. burnetii appears to be another example of an intracellular parasite with morphologically distinct developmental forms whose nucleoid structure may be controlled by histone H1 homologs.

Full Text

The Full Text of this article is available as a PDF (449.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barry C. E., 3rd, Brickman T. J., Hackstadt T. Hc1-mediated effects on DNA structure: a potential regulator of chlamydial development. Mol Microbiol. 1993 Jul;9(2):273–283. doi: 10.1111/j.1365-2958.1993.tb01689.x. [DOI] [PubMed] [Google Scholar]
  2. Barry C. E., 3rd, Hayes S. F., Hackstadt T. Nucleoid condensation in Escherichia coli that express a chlamydial histone homolog. Science. 1992 Apr 17;256(5055):377–379. doi: 10.1126/science.256.5055.377. [DOI] [PubMed] [Google Scholar]
  3. Brickman T. J., Barry C. E., 3rd, Hackstadt T. Molecular cloning and expression of hctB encoding a strain-variant chlamydial histone-like protein with DNA-binding activity. J Bacteriol. 1993 Jul;175(14):4274–4281. doi: 10.1128/jb.175.14.4274-4281.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Christiansen G., Pedersen L. B., Koehler J. E., Lundemose A. G., Birkelund S. Interaction between the Chlamydia trachomatis histone H1-like protein (Hc1) and DNA. J Bacteriol. 1993 Mar;175(6):1785–1795. doi: 10.1128/jb.175.6.1785-1795.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Deretic V., Konyecsni W. M. A procaryotic regulatory factor with a histone H1-like carboxy-terminal domain: clonal variation of repeats within algP, a gene involved in regulation of mucoidy in Pseudomonas aeruginosa. J Bacteriol. 1990 Oct;172(10):5544–5554. doi: 10.1128/jb.172.10.5544-5554.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hackstadt T., Baehr W., Ying Y. Chlamydia trachomatis developmentally regulated protein is homologous to eukaryotic histone H1. Proc Natl Acad Sci U S A. 1991 May 1;88(9):3937–3941. doi: 10.1073/pnas.88.9.3937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hackstadt T., Brickman T. J., Barry C. E., 3rd, Sager J. Diversity in the Chlamydia trachomatis histone homologue Hc2. Gene. 1993 Sep 30;132(1):137–141. doi: 10.1016/0378-1119(93)90526-9. [DOI] [PubMed] [Google Scholar]
  8. Hackstadt T. Purification and N-terminal amino acid sequences of Chlamydia trachomatis histone analogs. J Bacteriol. 1991 Nov;173(21):7046–7049. doi: 10.1128/jb.173.21.7046-7049.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kato J., Misra T. K., Chakrabarty A. M. AlgR3, a protein resembling eukaryotic histone H1, regulates alginate synthesis in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 1990 Apr;87(8):2887–2891. doi: 10.1073/pnas.87.8.2887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. McCaul T. F., Williams J. C. Developmental cycle of Coxiella burnetii: structure and morphogenesis of vegetative and sporogenic differentiations. J Bacteriol. 1981 Sep;147(3):1063–1076. doi: 10.1128/jb.147.3.1063-1076.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Miskimins W. K., Roberts M. P., McClelland A., Ruddle F. H. Use of a protein-blotting procedure and a specific DNA probe to identify nuclear proteins that recognize the promoter region of the transferrin receptor gene. Proc Natl Acad Sci U S A. 1985 Oct;82(20):6741–6744. doi: 10.1073/pnas.82.20.6741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Moulder J. W. Comparative biology of intracellular parasitism. Microbiol Rev. 1985 Sep;49(3):298–337. doi: 10.1128/mr.49.3.298-337.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Panyim S., Chalkley R. The molecular weights of vertebrate histones exploiting a modified sodium dodecyl sulfate electrophoretic method. J Biol Chem. 1971 Dec 25;246(24):7557–7560. [PubMed] [Google Scholar]
  15. Pearson W. R., Lipman D. J. Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444–2448. doi: 10.1073/pnas.85.8.2444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Perara E., Ganem D., Engel J. N. A developmentally regulated chlamydial gene with apparent homology to eukaryotic histone H1. Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2125–2129. doi: 10.1073/pnas.89.6.2125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Scarlato V., Aricò B., Goyard S., Ricci S., Manetti R., Prugnola A., Manetti R., Polverino-De-Laureto P., Ullmann A., Rappuoli R. A novel chromatin-forming histone H1 homologue is encoded by a dispensable and growth-regulated gene in Bordetella pertussis. Mol Microbiol. 1995 Mar;15(5):871–881. doi: 10.1111/j.1365-2958.1995.tb02357.x. [DOI] [PubMed] [Google Scholar]
  18. Silva C. M., Tully D. B., Petch L. A., Jewell C. M., Cidlowski J. A. Application of a protein-blotting procedure to the study of human glucocorticoid receptor interactions with DNA. Proc Natl Acad Sci U S A. 1987 Apr;84(7):1744–1748. doi: 10.1073/pnas.84.7.1744. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tao S., Kaul R., Wenman W. M. Identification and nucleotide sequence of a developmentally regulated gene encoding a eukaryotic histone H1-like protein from Chlamydia trachomatis. J Bacteriol. 1991 May;173(9):2818–2822. doi: 10.1128/jb.173.9.2818-2822.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]
  21. Wagar E. A., Stephens R. S. Developmental-form-specific DNA-binding proteins in Chlamydia spp. Infect Immun. 1988 Jul;56(7):1678–1684. doi: 10.1128/iai.56.7.1678-1684.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wiebe M. E., Burton P. R., Shankel D. M. Isolation and characterization of two cell types of Coxiella burneti phase I. J Bacteriol. 1972 Apr;110(1):368–377. doi: 10.1128/jb.110.1.368-377.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES