Figure 3.
Effect of in vivo exposure to ES-62 on the BCR-induced proliferative responses of lymph node cells and purified splenic B cells, ex vivo. Mice were exposed to PBS or ES-62 in vivo by release from osmotic pumps for two weeks. Mononuclear cells from lymph nodes (a) and purified splenic B cells (b) were stimulated for 48 hr in vitro in the presence of 50 µg/ml F(ab′)2 fragments of anti-mouse IgM as indicated. Cells cultured in the presence of medium alone were included as a control. Culture wells were pulsed with [3H]thymidine 4 hr prior to harvesting and [3H]thymidine-incorporation was assessed by scintillation counting. Data are the mean ± SD of triplicate measurements and are representative of at least two independent experiments. Significant differences are illustrated as ***P < 0·001 compared with control PBS levels.