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. 2003 Aug;109(4):572–579. doi: 10.1046/j.1365-2567.2003.01694.x

Figure 7.

Figure 7

The competition between the unlabeled and radiolabelled probes and probe supershift. (a) The radiolabelled AP-1 probes were incubated with nuclear extracts of U937 cells, which were treated without (control, C) or with 150 µg/ml of LDL-IC (IC), in the presence or absence of 50-fold unlabelled AP-1, SP-1 or NFκB probes. The protein–DNA complexes were detected by EMSA. (b) The radiolabelled AP-1 probes were incubated with nuclear extracts of U937 cells, which were treated with 150 µg/ml of LDL-IC, in the presence of absence of anti-c-jun antibodies. The protein–DNA–antibody complexes were detected by EMSA as supershift. (c) The radiolabelled Ets probes were incubated with nuclear extracts of U937 cells, which were treated without (control, C) or with 150 µg/ml of LDL-IC (IC), in the presence or absence of 50-fold unlabeled Ets and SP-1 probes. The protein–DNA complexes were detected by EMSA.