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. 2003 Nov;110(3):358–367. doi: 10.1046/j.1365-2567.2003.01744.x

Figure 1.

Figure 1

Proliferation of thymocytes in response to stimulation with anti-CD28 mAb, ionomycin and phorbol 12-myristate 13-acetate (PMA). (a) Proliferation of thymocytes from E18 embryos and D1 chicks following stimulation with different PMA concentrations for 24 and 48 hr. Mean counts per minute (c.p.m.) for thymocytes from the E18 embyros at 24 hr were 177 c.p.m. (unstimulated), 1839 c.p.m. (200 ng PMA/ml), 504 c.p.m. (500 ng PMA/ml), 957 c.p.m. (800 ng PMA/ml) and 741 c.p.m. (1500 ng PMA/ml), and from the E18 embyros at 48 hr were 48 c.p.m. (unstimulated), 384 c.p.m. (200 ng PMA/ml), 92 c.p.m. (500 ng PMA/ml), 143 c.p.m. (800 ng PMA/ml) and 121 c.p.m. (1500 ng PMA/ml). Mean c.p.m. for thymocytes from the D1 chicks at 24 hr were 1758 c.p.m. (unstimulated), 2080 c.p.m. (200 ng PMA/ml), 2429 c.p.m. (500 ng PMA/ml), 2243 c.p.m. (800 ng PMA/ml) and 1977 c.p.m. (1500 ng PMA/ml), and from the D1 chicks at 48 hr were 182 c.p.m. (unstimulated), 516 c.p.m. (200 ng PMA/ml), 391 c.p.m. (500 ng PMA/ml), 821 c.p.m. (800 ng PMA/ml) and 594 c.p.m. (1500 ng PMA/ml). (b) Effect of developmental stage on the ability of thymocytes to proliferate following stimulation with 200 ng PMA/ml for 24 and 48 hr. Mean c.p.m. for thymocytes from the E14 embyros at 24 hr were 9722 c.p.m. (unstimulated) and 6815 c.p.m. (200 ng PMA/ml), and from the E14 embyros at 48 hr were 5146 c.p.m. (unstimulated) and 5774 c.p.m. (200 ng PMA/ml). Mean c.p.m. for thymocytes from the E18 embyros at 24 hr were 139 c.p.m. (unstimulated), 780 c.p.m. (200 ng PMA/ml), and from the E18 embyros at 48 hr were 875 c.p.m. (unstimulated) and 981 c.p.m. (200 ng PMA/ml). Mean c.p.m. for thymocytes from the D1 chicks at 24 hr were 163 c.p.m. (unstimulated) and 780 c.p.m. (200 ng PMA/ml), and from the D1 chicks at 48 hr were 875 c.p.m. (unstimulated) and 981 c.p.m. (200 ng PMA/ml). Proliferation at 24 hr is shown as unshaded columns, and at 48 hr as shaded columns. (c) Proliferation of CD4+ TCR+ and CD8+ TCR+ thymocyte subsets from E18 embryos stimulated with 200 ng PMA/ml for 24 hr. Thymocytes with a double-positive phenotype are present in both populations. Mean c.p.m. for the CD4+ TCR+ thymocytes were 148 c.p.m. (unstimulated) and 736 c.p.m. (200 ng PMA/ml), for the CD4+ TCR+ thymocytes were 403 cpm (unstimulated) and 607 c.p.m. (200 ng PMA/ml), and for unsorted thymocytes were 150 c.p.m. (unstimulated) and 828 c.p.m. (200 ng PMA/ml).