Table 2.

Enhanced superoxide production in phorbol 12-myristate 13-acetate (PMA)-stimulated peritoneal macrophages (Mφ) from tartrate-resistant acid phosphatase (TRAP)-deficient and age- and gender-matched congenic wild-type mice

	TRAP-deficient	Wild-type
Basal	3·12 ± 0·87**	0·73 ± 0·26
LPS	9·69 ± 1·3*	4·65 ± 0·59
IFN-γ	8·25 ± 1·85	4·56 ± 0·85

Resident peritoneal Mφ were isolated and stimulated alone or with lipopolysaccharide (LPS) (1 µg/10⁶ cells) or interferon-γ (IFN-γ) (100 U/10⁶ cells) for 48 hr. Sixty minutes before harvesting, cells were stimulated with PMA (1 µg/10⁶ cells) and ferricytochrome c was added (80 µm). Superoxide, determined as described in the Materials and Methods, is shown as µmol of cytochrome c reduced/10⁶ cells/hr. Results are representative of two separate experiments, each using 10 determinations for each condition, and are expressed as mean±SEM.

^*P < 0·01;

^**P < 0·05.