Table 1. Vaccination trial.
Group (n = 5) | Plasmid | Vaccination route* | Dose (µg) | C. psittaci challenge§ |
---|---|---|---|---|
1 | pMOMP | i.m.† + i.n.‡ | 100 + 100 | Serovar A |
2 | pMOMP | i.m. | 100 | Serovar A |
3 | pMOMP | i.n. | 100 | Serovar A |
4 | pMOMP | Aerosol | 100 | Serovar A |
5 | pcDNA1 placebo | i.m. + i.n. | 100 + 100 | Serovar A |
6 | pMOMP | i.m. + i.n. | 100 + 100 | No |
7 | pMOMP+pIFN-γ | i.m. | 100 + 100 | Serovar A |
8 | pcDNA1 placebo+pCIneo placebo | i.m. | 100 + 100 | Serovar A |
9 | pcDNA1 placebo+pIFN-γ | i.m. | 100 + 100 | Serovar A |
10 | pMOMP+pIFN-γi.m. | 100 + 100 | No |
Each group received a primary vaccination at time 0 followed by a booster vaccination 3 weeks later.
i.m., intramuscular.
i.n. intranasal (DNA drops).
Challenge (104 50% tissue culture infective dose [TCID50] Chlamydophila psittaci 84/55 serovar A) was administered by aerosol at 14 days after the second DNA inoculation.
IFN-γ, interferon-γ.