Figure 6.

Epitope specificities of interferon-γ (IFN-γ)- and interleukin-4 (IL-4)-secreting cells induced in vivo by injection with hen-egg lysozyme (HEL) derivatives. Three groups of two BALB/c mice were immunized with 6,127CM-HEL, native HEL, or 35–108CL-HEL (50 µg/mouse) in complete Freund's adjuvant (CFA). Nine days later, spleen cells were cultured in the presence of 3 µm of HEL_8–35, HEL_98–116, or no antigen, at 10⁶ cells/well in anti-IL-4 antibody- or anti-IFN-γ antibody-precoated plates. After 48 hr of incubation, cytokine production was measured, using a sandwich enzyme-linked immunosorbent assay (ELISA), as described in the legend to Figure 5.