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. 1996 Sep;178(17):5316–5319. doi: 10.1128/jb.178.17.5316-5319.1996

Dynamic aspects of colicin N translocation through the Escherichia coli outer membrane.

R El Kouhen 1, J M Pagès 1
PMCID: PMC178333  PMID: 8752354

Abstract

Colicin N is a bacteriocin that kills sensitive Escherichia coli cells. After binding to the cell surface-exposed receptor, a short period exists when a significant number of the cell-associated colicin N molecules are sensitive to external enzymes. Two colicin N populations are discriminated by proteases: the susceptible pool bound to OmpF porin on the cell surface and another population corresponding to protease-inaccessible colicin N. During translocation, colicin N reaches the periplasmic space and proteolytic cleavage of the colicin occurs only when the outer membrane barrier is permeabilized.

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Selected References

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