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. 1996 Sep;178(18):5533–5536. doi: 10.1128/jb.178.18.5533-5536.1996

Positions of strand exchange in mycobacteriophage L5 integration and characterization of the attB site.

C E Peña 1, J E Stoner 1, G F Hatfull 1
PMCID: PMC178380  PMID: 8808947

Abstract

Mycobacteriophage L5 integrates into the genome of Mycobacterium smegmatis via site-specific recombination between the phage attP site and the bacterial attB site. These two sites have a 43-bp common core sequence within which strand exchange occurs and which overlaps a tRNAGly gene at attB. We show here that a 29-bp segment of DNA is necessary and sufficient for attB function and identify the positions of strand exchange.

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Selected References

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