Abstract
In the absence of fixation of ammonium to glutamine, nitrate and nitrite activated transcription of the nitrate assimilation (nirA-nrtABCD-narB) operon of Synechococcus sp. strain PCC 7942. In a nitrate reductase-deficient mutant, only nitrite activated transcription, indicating that nitrite is the actual activator of the operon. Nitrate and nitrite were also found to activate the transcription of a nitrate assimilation operon in the filamentous nonheterocystous nitrogen-fixing cyanobacterium Plectonema boryanum.
Full Text
The Full Text of this article is available as a PDF (565.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aiba H., Adhya S., de Crombrugghe B. Evidence for two functional gal promoters in intact Escherichia coli cells. J Biol Chem. 1981 Nov 25;256(22):11905–11910. [PubMed] [Google Scholar]
- Bender R. A., Friedrich B. Regulation of assimilatory nitrate reductase formation in Klebsiella aerogenes W70. J Bacteriol. 1990 Dec;172(12):7256–7259. doi: 10.1128/jb.172.12.7256-7259.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cali B. M., Micca J. L., Stewart V. Genetic regulation of nitrate assimilation in Klebsiella pneumoniae M5al. J Bacteriol. 1989 May;171(5):2666–2672. doi: 10.1128/jb.171.5.2666-2672.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Church G. M., Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. doi: 10.1073/pnas.81.7.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crawford N. M., Arst H. N., Jr The molecular genetics of nitrate assimilation in fungi and plants. Annu Rev Genet. 1993;27:115–146. doi: 10.1146/annurev.ge.27.120193.000555. [DOI] [PubMed] [Google Scholar]
- Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
- Goldman B. S., Lin J. T., Stewart V. Identification and structure of the nasR gene encoding a nitrate- and nitrite-responsive positive regulator of nasFEDCBA (nitrate assimilation) operon expression in Klebsiella pneumoniae M5al. J Bacteriol. 1994 Aug;176(16):5077–5085. doi: 10.1128/jb.176.16.5077-5085.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hawker K. L., Montague P., Kinghorn J. R. Nitrate reductase and nitrite reductase transcript levels in various mutants of Aspergillus nidulans: confirmation of autogenous regulation. Mol Gen Genet. 1992 Feb;231(3):485–488. doi: 10.1007/BF00292720. [DOI] [PubMed] [Google Scholar]
- Herrero A., Flores E., Guerrero M. G. Regulation of nitrate reductase levels in the cyanobacteria Anacystis nidulans, Anabaena sp. strain 7119, and Nostoc sp. strain 6719. J Bacteriol. 1981 Jan;145(1):175–180. doi: 10.1128/jb.145.1.175-180.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuhlemeier C. J., Logtenberg T., Stoorvogel W., van Heugten H. A., Borrias W. E., van Arkel G. A. Cloning of nitrate reductase genes from the cyanobacterium Anacystis nidulans. J Bacteriol. 1984 Jul;159(1):36–41. doi: 10.1128/jb.159.1.36-41.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lara C., Romero J. M., Guerrero M. G. Regulated nitrate transport in the cyanobacterium Anacystis nidulans. J Bacteriol. 1987 Sep;169(9):4376–4378. doi: 10.1128/jb.169.9.4376-4378.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Luque I., Flores E., Herrero A. Molecular mechanism for the operation of nitrogen control in cyanobacteria. EMBO J. 1994 Jun 15;13(12):2862–2869. doi: 10.1002/j.1460-2075.1994.tb06580.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Luque I., Flores E., Herrero A. Nitrite reductase gene from Synechococcus sp. PCC 7942: homology between cyanobacterial and higher-plant nitrite reductases. Plant Mol Biol. 1993 Mar;21(6):1201–1205. doi: 10.1007/BF00023618. [DOI] [PubMed] [Google Scholar]
- Marco E., Ohad N., Schwarz R., Lieman-Hurwitz J., Gabay C., Kaplan A. High CO2 concentration alleviates the block in photosynthetic electron transport in an ndhB-inactivated mutant of Synechococcus sp. PCC 7942. Plant Physiol. 1993 Mar;101(3):1047–1053. doi: 10.1104/pp.101.3.1047. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marzluf G. A. Regulation of sulfur and nitrogen metabolism in filamentous fungi. Annu Rev Microbiol. 1993;47:31–55. doi: 10.1146/annurev.mi.47.100193.000335. [DOI] [PubMed] [Google Scholar]
- Omata T., Andriesse X., Hirano A. Identification and characterization of a gene cluster involved in nitrate transport in the cyanobacterium Synechococcus sp. PCC7942. Mol Gen Genet. 1993 Jan;236(2-3):193–202. doi: 10.1007/BF00277112. [DOI] [PubMed] [Google Scholar]
- Omata T., Ohmori M., Arai N., Ogawa T. Genetically engineered mutant of the cyanobacterium Synechococcus PCC 7942 defective in nitrate transport. Proc Natl Acad Sci U S A. 1989 Sep;86(17):6612–6616. doi: 10.1073/pnas.86.17.6612. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ramos F., Blanco G., Gutiérrez J. C., Luque F., Tortolero M. Identification of an operon involved in the assimilatory nitrate-reducing system of Azotobacter vinelandii. Mol Microbiol. 1993 Jun;8(6):1145–1153. doi: 10.1111/j.1365-2958.1993.tb01659.x. [DOI] [PubMed] [Google Scholar]
- Stanier R. Y., Kunisawa R., Mandel M., Cohen-Bazire G. Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev. 1971 Jun;35(2):171–205. doi: 10.1128/br.35.2.171-205.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Suzuki I., Kikuchi H., Nakanishi S., Fujita Y., Sugiyama T., Omata T. A novel nitrite reductase gene from the cyanobacterium Plectonema boryanum. J Bacteriol. 1995 Nov;177(21):6137–6143. doi: 10.1128/jb.177.21.6137-6143.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Suzuki I., Sugiyama T., Omata T. Regulation of Nitrite Reductase Activity under CO2 Limitation in the Cyanobacterium Synechococcus sp. PCC7942. Plant Physiol. 1995 Mar;107(3):791–796. doi: 10.1104/pp.107.3.791. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Suzuki I., Sugiyami T., Omata T. Regulation by cyanate of the genes involved in carbon and nitrogen assimilation in the cyanobacterium Synechococcus sp. strain PCC 7942. J Bacteriol. 1996 May;178(9):2688–2694. doi: 10.1128/jb.178.9.2688-2694.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Unkles S. E., Hawker K. L., Grieve C., Campbell E. I., Montague P., Kinghorn J. R. crnA encodes a nitrate transporter in Aspergillus nidulans. Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):204–208. doi: 10.1073/pnas.88.1.204. [DOI] [PMC free article] [PubMed] [Google Scholar]