Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1996 Oct;178(19):5826–5830. doi: 10.1128/jb.178.19.5826-5830.1996

Rgg is a positive transcriptional regulator of the Streptococcus gordonii gtfG gene.

M C Sulavik 1, D B Clewell 1
PMCID: PMC178431  PMID: 8824637

Abstract

The Streptococcus gordonii (Challis) glucosyltransferase-encoding determinant gtfG is regulated by the product of the adjacent gene rgg. Results of analyses described here showed that in both S. gordonii and Escherichia coli Rgg is a positive transcriptional regulator of glucosyltransferase expression. In addition, the transcriptional start sites of both gtfG and rgg were determined.

Full Text

The Full Text of this article is available as a PDF (633.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAILEY R. W. Transglucosidase activity of rumen strains of Streptococcus bovis. 2. Isolation and properties of dextransucrase. Biochem J. 1959 May;72(1):42–49. doi: 10.1042/bj0720042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carlsson J., Elander B. Regulation of dextransucrase formation by Streptococcus sanguis. Caries Res. 1973;7(2):89–101. doi: 10.1159/000259834. [DOI] [PubMed] [Google Scholar]
  3. Haisman R. J., Jenkinson H. F. Mutants of Streptococcus gordonii Challis over-producing glucosyltransferase. J Gen Microbiol. 1991 Mar;137(3):483–489. doi: 10.1099/00221287-137-3-483. [DOI] [PubMed] [Google Scholar]
  4. Harley C. B., Lawrie J., Boyer H. W., Hedgpeth J. Reiterative copying by E.coli RNA polymerase during transcription initiation of mutant pBR322 tet promoters. Nucleic Acids Res. 1990 Feb 11;18(3):547–552. doi: 10.1093/nar/18.3.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Huang F., Coppola G., Calhoun D. H. Multiple transcripts encoded by the ilvGMEDA gene cluster of Escherichia coli K-12. J Bacteriol. 1992 Aug;174(15):4871–4877. doi: 10.1128/jb.174.15.4871-4877.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hudson M. C., Curtiss R., 3rd Regulation of expression of Streptococcus mutans genes important to virulence. Infect Immun. 1990 Feb;58(2):464–470. doi: 10.1128/iai.58.2.464-470.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Keevil C. W., West A. A., Bourne N., Marsh P. D. Inhibition of the synthesis and secretion of extracellular glucosyl- and fructosyltransferase in Streptococcus sanguis by sodium ions. J Gen Microbiol. 1984 Jan;130(1):77–82. doi: 10.1099/00221287-130-1-77. [DOI] [PubMed] [Google Scholar]
  8. Loesche W. J. Role of Streptococcus mutans in human dental decay. Microbiol Rev. 1986 Dec;50(4):353–380. doi: 10.1128/mr.50.4.353-380.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Murakawa G. J., Kwan C., Yamashita J., Nierlich D. P. Transcription and decay of the lac messenger: role of an intergenic terminator. J Bacteriol. 1991 Jan;173(1):28–36. doi: 10.1128/jb.173.1.28-36.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ramirez-Ronda C. H. Adherence of glucan-positive and glucan-negative streptococcal strains to normal and damaged heart valves. J Clin Invest. 1978 Oct;62(4):805–814. doi: 10.1172/JCI109192. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sato M., Tsuchiya H., Kato M., Yamamoto K., Nakazato G., Takagi N., Namikawa I. Effects of Tween 80 and sodium fluoride on extracellular glucosyltransferase production and membrane lipids of Streptococcus mutans. Int J Biochem. 1989;21(7):751–754. doi: 10.1016/0020-711x(89)90206-1. [DOI] [PubMed] [Google Scholar]
  13. Scheld W. M., Valone J. A., Sande M. A. Bacterial adherence in the pathogenesis of endocarditis. Interaction of bacterial dextran, platelets, and fibrin. J Clin Invest. 1978 May;61(5):1394–1404. doi: 10.1172/JCI109057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shaw J. H., Clewell D. B. Complete nucleotide sequence of macrolide-lincosamide-streptogramin B-resistance transposon Tn917 in Streptococcus faecalis. J Bacteriol. 1985 Nov;164(2):782–796. doi: 10.1128/jb.164.2.782-796.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Simons R. W., Houman F., Kleckner N. Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene. 1987;53(1):85–96. doi: 10.1016/0378-1119(87)90095-3. [DOI] [PubMed] [Google Scholar]
  16. Su Y. A., Sulavik M. C., He P., Makinen K. K., Makinen P. L., Fiedler S., Wirth R., Clewell D. B. Nucleotide sequence of the gelatinase gene (gelE) from Enterococcus faecalis subsp. liquefaciens. Infect Immun. 1991 Jan;59(1):415–420. doi: 10.1128/iai.59.1.415-420.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sulavik M. C., Tardif G., Clewell D. B. Identification of a gene, rgg, which regulates expression of glucosyltransferase and influences the Spp phenotype of Streptococcus gordonii Challis. J Bacteriol. 1992 Jun;174(11):3577–3586. doi: 10.1128/jb.174.11.3577-3586.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tardif G., Sulavik M. C., Jones G. W., Clewell D. B. Spontaneous switching of the sucrose-promoted colony phenotype in Streptococcus sanguis. Infect Immun. 1989 Dec;57(12):3945–3948. doi: 10.1128/iai.57.12.3945-3948.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Umesaki Y., Kawai Y., Mutai M. Effect of Tween 80 on glucosyltransferase production in Streptococcus mutans. Appl Environ Microbiol. 1977 Aug;34(2):115–119. doi: 10.1128/aem.34.2.115-119.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vickerman M. M., Clewell D. B., Jones G. W. Sucrose-promoted accumulation of growing glucosyltransferase variants of Streptococcus gordonii on hydroxyapatite surfaces. Infect Immun. 1991 Oct;59(10):3523–3530. doi: 10.1128/iai.59.10.3523-3530.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wells V. D., Munro C. L., Sulavik M. C., Clewell D. B., Macrina F. L. Infectivity of a glucan synthesis-defective mutant of Streptococcus gordonii (Challis) in a rat endocarditis model. FEMS Microbiol Lett. 1993 Sep 15;112(3):301–305. doi: 10.1111/j.1574-6968.1993.tb06466.x. [DOI] [PubMed] [Google Scholar]
  22. White J. C., Niven C. F., Jr Streptococcus S.B.E.: A Streptococcus Associated with Subacute Bacterial Endocarditis. J Bacteriol. 1946 Jun;51(6):717–722. doi: 10.1128/jb.51.6.717-722.1946. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES